Inactivation of Escherichia coli O157:H7 Attached to Spinach Harvester Blade Using Bacteriophage
| العنوان: | Inactivation of Escherichia coli O157:H7 Attached to Spinach Harvester Blade Using Bacteriophage |
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| المؤلفون: | Manan Sharma, Patricia Millner, Jitendra Patel, Manpreet Singh, Todd Calaway |
| المصدر: | Foodborne Pathogens and Disease. 8:541-546 |
| بيانات النشر: | Mary Ann Liebert Inc, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Crops, Agricultural, Time Factors, Colony Count, Microbial, Escherichia coli O157, medicine.disease_cause, Applied Microbiology and Biotechnology, Microbiology, Bacterial Adhesion, Tryptic soy broth, Foodborne Diseases, Bacteriophage, chemistry.chemical_compound, Species Specificity, Spinacia oleracea, medicine, Food microbiology, Bacteriophages, Food science, Pest Control, Biological, Incubation, Escherichia coli, Microbial Viability, biology, Plant Extracts, Inoculation, Temperature, food and beverages, Agriculture, biology.organism_classification, Plant Leaves, chemistry, Biofilms, Food Microbiology, Colony count, Spinach, Animal Science and Zoology, Food Science |
| الوصف: | Outbreaks associated with leafy greens have focused attention on the transfer of human pathogens to these commodities during harvest with commercial equipment. Attachment of Escherichia coli O157:H7 on new or rusty spinach harvester blades immersed in spinach extract or 10% tryptic soy broth (TSB) was investigated. Bacteriophages specific for E. coli O157:H7 were evaluated to kill cells attached to blade. A cocktail of five nalidixic acid-resistant E. coli O157:H7 isolates was transferred to 25 mL of spinach extract or 10% TSB. A piece of sterilized spinach harvester blade (2×1") was placed in above spinach extract or 10% TSB and incubated at room (22 °C) or dynamic (30 °C day, 20 °C night) temperatures. E. coli O157:H7 populations attached to blade during incubation in spinach extract or 10% TSB were determined. When inoculated at 1 log CFU/mL, E. coli O157:H7 attachment to blades after 24 and 48 h incubation at dynamic temperature (6.09 and 6.37 log CFU/mL) was significantly higher than when incubated at 22 °C (4.84 and 5.68 log CFU/mL), respectively. After 48 h incubation, two blades were sprayed on each side with a cocktail of E. coli O157-specific bacteriophages before scraping the blade, and subsequent plating on Sorbitol MacConkey media-nalidixic acid. Application of bacteriophages reduced E. coli O157:H7 populations by 4.5 log CFU on blades after 2 h of phage treatment. Our study demonstrates that E. coli O157:H7 can attach to and proliferate on spinach harvester blades under static and dynamic temperature conditions, and bacteriophages are able to reduce E. coli O157:H7 populations adhered to blades. |
| تدمد: | 1556-7125 1535-3141 |
| DOI: | 10.1089/fpd.2010.0734 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5543dc2ab0e6e9c4ff1484a444b0f3f6 https://doi.org/10.1089/fpd.2010.0734 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....5543dc2ab0e6e9c4ff1484a444b0f3f6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15567125 15353141 |
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| DOI: | 10.1089/fpd.2010.0734 |