MICROSATELLITE MARKERS DEVELOPED FOR UTRICULARIA RENIFORMIS (LENTIBULARIACEAE)
| العنوان: | MICROSATELLITE MARKERS DEVELOPED FOR UTRICULARIA RENIFORMIS (LENTIBULARIACEAE) |
|---|---|
| المؤلفون: | Welington Luiz Araújo, Vitor F. O. Miranda, Débora Clivati, Alexandre Wagner Silva Hilsdorf, Matthew A. Gitzendanner |
| المساهمون: | Univ Mogi das Cruzes, Univ Florida, Universidade de São Paulo (USP), Universidade Estadual Paulista (Unesp) |
| المصدر: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| بيانات النشر: | Botanical Soc Amer Inc, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Lentibulariaceae, Population, Plant Science, Biology, Gene Frequency, Species Specificity, Genetic variation, Botany, Genetics, education, Ecology, Evolution, Behavior and Systematics, Alleles, DNA Primers, Utricularia, Genetic diversity, education.field_of_study, Lamiaceae, Genetic Variation, MICROBIOLOGIA, genetic diversity, biology.organism_classification, bladderworts, Genetics, Population, Genetic Loci, Pinguicula, Atlantic Forest, Microsatellite, endemic plants, Utricularia reniformis, Brazil, Microsatellite Repeats |
| الوصف: | Made available in DSpace on 2013-08-12T18:37:09Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-09-01 Made available in DSpace on 2013-09-30T19:11:46Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-09-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:30:30Z No. of bitstreams: 0 Made available in DSpace on 2014-05-20T15:30:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-09-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Premise of the study: Microsatellite markers were developed to study the genetic diversity and population structure of the carnivorous bladderwort Utricularia reniformis, which is endemic to the Atlantic Forest of southern and southeastern Brazil. Cross-species amplification was tested in U. gibba, U. neottioides, U. subulata, and Pinguicula benedicta.Methods and Results: The U. reniformis genome was sequenced in a 454 GS FLX sequencer, and eight primer sets were developed based on the microsatellites identified from the reads. All loci are polymorphic, showing 1.6 to 4.8 alleles per population. Preliminary results show that primer sets are suitable for population-level studies. Cross-species amplifi cation was successful in three other Utricularia species and one Pinguicula species.Conclusions: Markers developed in this study provide tools for analyses of intra- and interpopulation genetic diversity in Utricularia and Pinguicula. Univ Mogi das Cruzes, Nucleo Integrado Biotecnol, BR-08780911 São Paulo, Brazil Univ Florida, Dept Biol, Gainesville, FL 32611 USA Univ São Paulo, Dept Microbiol, BR-05508900 São Paulo, Brazil Univ Estadual Paulista, Fac Ciencias Agr & Vet, BR-14884900 São Paulo, Brazil Univ Estadual Paulista, Fac Ciencias Agr & Vet, BR-14884900 São Paulo, Brazil FAPESP: 08/22239-9 FAPESP: 07/58277-7 CAPES: 5316/10-3 |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::53bb8405967961071dcb5f0c22c07765 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....53bb8405967961071dcb5f0c22c07765 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |