Hemijske modifikacije Art v 1, glavnog alergena Artemisia vulgaris, cis-akonitilovanjem i citrakonilovanjem
| العنوان: | Hemijske modifikacije Art v 1, glavnog alergena Artemisia vulgaris, cis-akonitilovanjem i citrakonilovanjem |
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| المؤلفون: | Lidija Burazer, Dragana Stanic, Marija Gavrović-Jankulović, Ratko M. Jankov, Tanja Ćirković-Veličković |
| المصدر: | Journal of the Serbian Chemical Society Journal of the Serbian Chemical Society, Vol 74, Iss 4, Pp 359-366 (2009) |
| بيانات النشر: | National Library of Serbia, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Art v 1, medicine.disease_cause, lcsh:Chemistry, Acylation, 03 medical and health sciences, Hydrolysis, 0302 clinical medicine, Mugwort, Allergen, medicine, Organic chemistry, 030304 developmental biology, Artemisia vulgaris, 0303 health sciences, mugwort pollen, biology, Chemistry, art v 1, allergen-specific immunotherapy, Chemical modification, General Chemistry, biology.organism_classification, medicine.disease, Allergoid, lcsh:QD1-999, 030228 respiratory system, Hay fever, allergoid, chemical modification |
| الوصف: | Art v 1 is the major allergen of mugwort (Artemisia vulgaris) pollen, a significant cause of hay fever all over Europe. Specific immunotherapy is the only treatment modality for allergic disease. Application of modified allergens makes the treatment safer and more efficient. In this work, two out of three (citraconic anhydride, cis-aconitic anhydride, 2,3-dimethylmaleic anhydride) tested anhydrides were proven to be suitable for chemical modifications of allergens. Art v 1 was modified by cis-aconitylation and citraconylation in order to obtain derivatives of Art v 1 that may be suitable for further immunological testing. Acylation of Art v 1 gave derivatives (caaArt v 1 and citArt v 1) with about 80 % modified amino groups. The derivatives were in the monomeric form and had dramatically reduced pI values. Both derivatives were relatively stable at neutral pH values, while the acyl groups undergo hydrolysis under acidic conditions. Modification of allergens by cis-aconitylation and citraconylation could be a new tool for obtaining allergoids. Art v1 je glavni alergen polena crnog pelina (Artemisia vulgaris), značajnog uzročnika polenske groznice širom Evrope. Alergen-specifična imunoterapija je za sada jedini delotvoran način za tretiranje alergija, pri čemu primena modifikovanih alergena čini ovakav tretman bezbednijim i efikasnijim. U ovom radu, dva od tri (anhidrid cis-akonitne, citrakonske i 2,3-dimetilmaleinske kiseline) ispitivana anhidrida su se pokazala pogodnim za hemijske modifikacije alergena. Art v 1 je modifikovan cis-akonitilovanjem i citrakonilovanjem u cilju dobijanja derivata Art v 1 pogodnih za dalje imunološke testove. Acilovanjem Art v 1 dobijeni su derivati (caaArt v 1 i citArt v 1) sa oko 80 % izmodifikovanih amino grupa. Dobijeni derivati su monomerni, sa molekulskom masom sličnom nativnom Art v 1, ali sa dramatično smanjenim pI vrednostima. Oba derivata su relativno stabilna u neutralnoj, dok se u kiseloj sredini acil grupe hidrolizuju. Modifikacija alergena cis-akonitilovanjem i citrakonilovanjem može biti novi način za dobijanje alergoida. |
| تدمد: | 1820-7421 0352-5139 |
| DOI: | 10.2298/jsc0904359s |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::52b475dc81abe0fcedce6e3dc867a9ec https://doi.org/10.2298/jsc0904359s |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....52b475dc81abe0fcedce6e3dc867a9ec |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18207421 03525139 |
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| DOI: | 10.2298/jsc0904359s |