In VivoDetection of Reactive Oxygen Species and Redox Status inCaenorhabditis elegans
| العنوان: | In VivoDetection of Reactive Oxygen Species and Redox Status inCaenorhabditis elegans |
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| المؤلفون: | De Henau S, Bart P. Braeckman, Smolders A, Patricia Back |
| المصدر: | Antioxidants & Redox Signaling. 25:577-592 |
| بيانات النشر: | Mary Ann Liebert Inc, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Physiology, Green Fluorescent Proteins, Clinical Biochemistry, Biosensing Techniques, Biology, Biochemistry, Redox, Germline, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Superoxides, In vivo, Animals, Somatic gonad, Caenorhabditis elegans, Molecular Biology, General Environmental Science, chemistry.chemical_classification, Reactive oxygen species, Superoxide, Hydrogen Peroxide, Cell Biology, Forum Review Articles, biology.organism_classification, Redox status, Cell biology, Luminescent Proteins, 030104 developmental biology, Microscopy, Fluorescence, chemistry, General Earth and Planetary Sciences, Reactive Oxygen Species, Oxidation-Reduction, 030217 neurology & neurosurgery |
| الوصف: | Significance: Due to its large families of redox-active enzymes, genetic amenability, and complete transparency, the nematode Caenorhabditis elegans has the potential to become an important model for the in vivo study of redox biology. Recent Advances: The recent development of several genetically encoded ratiometric reactive oxygen species (ROS) and redox sensors has revolutionized the quantification and precise localization of ROS and redox signals in living organisms. Only few exploratory studies have applied these sensors in C. elegans and undoubtedly much remains to be discovered in this model. As a follow-up to our recent findings that the C. elegans somatic gonad uses superoxide and hydrogen peroxide (H2O2) signals to communicate with the germline, we here analyze the patterns of H2O2 inside the C. elegans germline. Critical Issues: Despite the advantages of genetically encoded ROS and redox sensors over classic chemical sensors, still several general as well as C. elegans-specific issues need to be addressed. The major concerns for the application of these sensors in C. elegans are (i) decreased vitality of some reporter strains, (ii) interference of autofluorescent compartments with the sensor signal, and (iii) the use of immobilization methods that do not influence the worm's redox physiology. Future Directions: We propose that several of the current issues may be solved by designing reporter strains carrying single copies of codon-optimized sensors. Preferably, these sensors should have their emission wavelengths in the red region, where autofluorescence is absent. Worm analysis could be optimized using four-dimensional ratiometric fluorescence microscopy of worms immobilized in microfluidic chips. Antioxid. Redox Signal. 25, 577–592. |
| تدمد: | 1557-7716 1523-0864 |
| DOI: | 10.1089/ars.2016.6751 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::51522ad2dc49b47b955f73317cdbcb4a https://doi.org/10.1089/ars.2016.6751 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....51522ad2dc49b47b955f73317cdbcb4a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15577716 15230864 |
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| DOI: | 10.1089/ars.2016.6751 |