Animal infections by vaccinia-like viruses in the state of Rio de Janeiro: an expanding disease
| العنوان: | Animal infections by vaccinia-like viruses in the state of Rio de Janeiro: an expanding disease |
|---|---|
| المؤلفون: | Renata Vitória Campos Costa, M. C. R. Gonçalves, Bruno R. Simonetti, José da Costa Farias-Filho, Ortrud Monika Barth, Manuel Enderson Vieira Silva, Paulo Sérgio D'Andrea, Hermann G. Schatzmayr, Marconny Gerhardt, José Pascoal Simonetti, Sandra Regina Rodrigues Simonetti, Danielle C Abreu |
| المصدر: | Pesquisa Veterinária Brasileira v.29 n.7 2009 Pesquisa Veterinária Brasileira Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA |
| بيانات النشر: | FapUNIFESP (SciELO), 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | electron microscopy, General Veterinary, biology, Inoculation, viruses, Orthopoxvirus infections, biology.organism_classification, Virology, Virus, state of Rio de Janeiro, chemistry.chemical_compound, PCR, Plaque reduction neutralization test, chemistry, biology.protein, Vero cell, Orthopoxvirus, Vaccinia, Antibody, neutralization test, Dairy cattle |
| الوصف: | In the present study we investigated the presence of infections by vaccinia-like viruses in dairy cattle from 12 counties in the state of Rio de Janeiro in the last 9 years. Clinical specimens were collected from adult animals with vesicular/pustular lesions mainly in the udder and teats, and from calves with lesions around the nose and mouth. A plaque reduction neutralization test (PRNT) was applied to search for antibodies to Orthopoxvirus; the vesicular/pustular fluids and scabs were examined by PCR, electron microscopy (EM) and by inoculation in VERO cells for virus isolation. Antibodies to Orthopoxvirus were detected in most cases. The PCR test indicated a high nucleotide homology among the isolates and the vaccinia viruses (VACV) used as controls. By EM, typical orthopoxvirus particles were observed in some specimens. The agents isolated in tissue culture were confirmed as vaccinia-like viruses by EM and PCR. The HA gene of the vaccinia-like Cantagalo/IOC virus isolated in our laboratory was sequenced and compared with other vaccinia-like isolates, showing high homology with the original Cantagalo strain, both strains isolated in 1999 from dairy cattle. Antibodies to Orthopoxvirus were detected in one wild rodent (genus Akodon sp.) collected in the northwestern region of the state, indicating the circulation of poxvirus in this area. Nonetheless, PCR applied to tissue samples collected from the wild rodents were negative. Vesicular/pustular lesions in people in close contact with animals have been also recorded. Thus, the vaccinia-like virus infections in cattle and humans in the state seem to be an expanding condition, resulting in economic losses to dairy herds and leading to transient incapacitating human disease. Therefore, a possible immunization of the dairy cattle in the state should be carefully evaluated. |
| وصف الملف: | text/html |
| تدمد: | 0100-736X |
| DOI: | 10.1590/s0100-736x2009000700004 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::514215fbed8148ae639e8549cfc43bce https://doi.org/10.1590/s0100-736x2009000700004 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....514215fbed8148ae639e8549cfc43bce |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 0100736X |
|---|---|
| DOI: | 10.1590/s0100-736x2009000700004 |