Blood plasma miR‐20a‐5p expression as a potential non‐invasive diagnostic biomarker of male infertility: A pilot study
| العنوان: | Blood plasma miR‐20a‐5p expression as a potential non‐invasive diagnostic biomarker of male infertility: A pilot study |
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| المؤلفون: | Andrea Cocci, Maria Elisabetta Coccia, Elisabetta Micelli, Pamela Pinzani, Alessandro Natali, Claudia Giachini, Francesca Salvianti, Gianmartin Cito, P. Verrienti, Rossella Fucci, Patrizia Falcone, Marco Carini, Rita Picone, Andrea Minervini |
| المصدر: | Andrology. 8:1256-1264 |
| بيانات النشر: | Wiley, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Adult, Infertility, Oncology, medicine.medical_specialty, Urology, Endocrinology, Diabetes and Metabolism, Pilot Projects, Semen, Male infertility, 03 medical and health sciences, 0302 clinical medicine, Endocrinology, Internal medicine, Blood plasma, medicine, Humans, Azoospermia, 030219 obstetrics & reproductive medicine, business.industry, Tubal factor infertility, medicine.disease, Testicular sperm extraction, MicroRNAs, Reproductive Medicine, Female, business, Spermatogenesis, Biomarkers |
| الوصف: | Background Recently, alterations in miRNAs expression profile in semen have been linked to damaged spermatogenesis, suggesting miRNAs could be used as potential infertility biomarkers. In previous animal studies, miR-20a-5p was found to be down-expressed in low motile spermatozoa, implying its potential target of genes associated with cell apoptosis. Objective To investigate miR-20a-5p expression in blood plasma of patients suffering from non-obstructive azoospermia (NOA), compared to normozoospermic controls. Materials and methods Between January 2018 and December 2019, from 52 infertile couples eligible for the study, 24 couples were finally enrolled in this monocentric observational prospective pilot study. Patients were included into two groups: Group 1 comprised men with NOA (n = 14) and Group 2 fertile men partners of women with female tubal factor infertility (n = 10). All NOA patients underwent testicular sperm extraction. The expression of circulating miR-20a-5p in plasma samples was assessed by RT-qPCR. A relative quantification strategy was adopted using the 2-ΔCq method to calculate the target miR-20a-5p expression with respect to miR-16-5p as endogenous control. Results Median blood plasma miR-20a-5p was significantly higher in patients affected by NOA (0.16 2-ΔCt , range: 0.05-0.79 2-ΔCt ) than in fertile controls (0.06 2-ΔCt , range: 0.04-0.10 2-ΔCt ), P .05). Conclusions MiR-20a-5p could represent a novel non-invasive diagnostic biomarker of male infertility. |
| تدمد: | 2047-2927 2047-2919 |
| DOI: | 10.1111/andr.12816 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::50f0f59a34632461da6f9a15a16582c0 https://doi.org/10.1111/andr.12816 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....50f0f59a34632461da6f9a15a16582c0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20472927 20472919 |
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| DOI: | 10.1111/andr.12816 |