Comparable Senescence Induction in Three-dimensional Human Cartilage Model by Exposure to Therapeutic Doses of X-rays or C-ions
| العنوان: | Comparable Senescence Induction in Three-dimensional Human Cartilage Model by Exposure to Therapeutic Doses of X-rays or C-ions |
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| المؤلفون: | Carl Mann, François Chevalier, Jean-Yves Thuret, Florent Durantel, Dounia Houria Hamdi, Yannick Saintigny, J.-E. Groetz |
| المساهمون: | Laboratoire d'Accueil et de Recherche avec les Ions Accélérés (LARIA), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Laboratoire Chrono-environnement (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Centre de recherche sur les Ions, les MAtériaux et la Photonique (CIMAP - UMR 6252), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche sur les Matériaux Avancés (IRMA), Normandie Université (NU)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Rouen Normandie (UNIROUEN), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Génétique moléculaire et destin cellulaire (FRE 3377), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Sénescence et stabilité génomique (SEN), Département Biologie des Génomes (DBG), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Normandie Université (NU)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Caen Normandie (UNICAEN), Normandie Université (NU), Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Sud - Paris 11 (UP11) |
| المصدر: | International Journal of Radiation Oncology, Biology, Physics International Journal of Radiation Oncology, Biology, Physics, 2016, 95 (1), pp.139-146. ⟨10.1016/j.ijrobp.2016.02.014⟩ International Journal of Radiation Oncology-Biology-Physics International Journal of Radiation Oncology-Biology-Physics, Elsevier, 2016, 95 (1), pp.139-146. ⟨10.1016/j.ijrobp.2016.02.014⟩ |
| بيانات النشر: | HAL CCSD, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Senescence, Pathology, medicine.medical_specialty, Cancer Research, Cell Survival, medicine.medical_treatment, Cell Culture Techniques, Chondrosarcoma, Linear energy transfer, Bone Neoplasms, Heavy Ion Radiotherapy, [SDV.CAN]Life Sciences [q-bio]/Cancer, Ion, 03 medical and health sciences, 0302 clinical medicine, Chondrocytes, Relative biological effectiveness, Medicine, Humans, Radiology, Nuclear Medicine and imaging, Linear Energy Transfer, Irradiation, Radiation Injuries, Cells, Cultured, Cellular Senescence, Particle therapy, Radiation, Cell Death, business.industry, Cartilage, X-Rays, Carbon, 3. Good health, Radiation therapy, 030104 developmental biology, medicine.anatomical_structure, Oncology, Radiology Nuclear Medicine and imaging, 030220 oncology & carcinogenesis, Biophysics, [PHYS.PHYS.PHYS-MED-PH]Physics [physics]/Physics [physics]/Medical Physics [physics.med-ph], business, Relative Biological Effectiveness |
| الوصف: | PurposeParticle therapy using carbon ions (C-ions) has been successfully used in the treatment of tumors resistant to conventional radiation therapy. However, the potential side effects to healthy cartilage exposed to lower linear energy transfer (LET) ions in the beam track before the tumor have not been evaluated. The aim of the present study was to assess the extent of damage after C-ion irradiation in a 3-dimensional (3D) cartilage model close to human homeostasis.Methods and MaterialsPrimary human articular chondrocytes from a healthy donor were cultured in a collagen scaffold to construct a physioxic 3D cartilage model. A 2-dimensional (2D) culture was used as a reference. The cells were irradiated with a single dose of a monoenergetic C-ion beam with a LET of approximatively 30 keV/μm. This LET corresponds to the entrance channel of C-ions in the shallow healthy tissues before the spread-out Bragg peak (∼100 keV/μm) during hadron therapy protocols. The same dose of X-rays was used as a reference. Survival, cell death, and senescence assays were performed.ResultsAs expected, in the 2D culture, C-ions were more efficient than X-rays in reducing cell survival with a relative biological effectiveness of 2.6. This correlated with stronger radiation-induced senescence (two-fold) but not with higher cell death induction. This differential effect was not reflected in the 3D culture. Both ionizing radiation types induced a comparable rate of senescence induction in the 3D model.ConclusionsThe greater biological effectiveness of C-ions compared with low LET radiation when evaluated in treatment planning systems might be misevaluated using 2D culture experiments. Radiation-induced senescence is an important factor of potential cartilage attrition. The present data should encourage the scientific community to use relevant models and beams to improve the use of charged particles with better safety for patients. |
| اللغة: | English |
| تدمد: | 0360-3016 1879-355X |
| DOI: | 10.1016/j.ijrobp.2016.02.014⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::50a420e2c3cd1facc64a642f7ea30614 https://cea.hal.science/cea-01938049 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....50a420e2c3cd1facc64a642f7ea30614 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 03603016 1879355X |
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| DOI: | 10.1016/j.ijrobp.2016.02.014⟩ |