In vitro immunomodulatory effects of microencapsulated umbilical cord Wharton jelly-derived mesenchymal stem cells in primary Sjögren’s syndrome
| العنوان: | In vitro immunomodulatory effects of microencapsulated umbilical cord Wharton jelly-derived mesenchymal stem cells in primary Sjögren’s syndrome |
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| المؤلفون: | Riccardo Calafiore, Giuseppe Basta, Onelia Bistoni, Vittorio Bini, Elena Bartoloni, Ilaria Pennoni, Alessia Alunno, Roberto Gerli, Teresa Pescara, Pia Montanucci, Sara Caterbi |
| المصدر: | Rheumatology. 54:163-168 |
| بيانات النشر: | Oxford University Press (OUP), 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Drug Compounding, T-Lymphocytes, T cell, Lymphocyte, Cell Communication, Lymphocyte proliferation, In Vitro Techniques, T-Lymphocytes, Regulatory, Peripheral blood mononuclear cell, Umbilical cord, Interferon-gamma, Drug Delivery Systems, Immune system, Rheumatology, Wharton's jelly, medicine, Humans, Immunologic Factors, Pharmacology (medical), Sjögren’s syndrome, Th17 cells, Treg cells, human umbilical cord mesenchymal cells, microencapsulation, Cell Proliferation, business.industry, Mesenchymal stem cell, Mesenchymal Stem Cells, Coculture Techniques, stomatognathic diseases, Phenotype, Sjogren's Syndrome, medicine.anatomical_structure, Case-Control Studies, Immunology, Cytokines, Female, business |
| الوصف: | Human umbilical cord Wharton jelly-derived mesenchymal stem cells (hUCMS) are easy to retrieve in bulk. They may interact with immune cells by either cell contact or soluble factors. Little evidence is currently available on potential therapeutic application of hUCMS to systemic autoimmune disorders such as primary SS (pSS). We have recently developed an endotoxin-free alginate gel that can be used to microencapsulate different cell types for graft into non-immunosuppressed hosts. We aimed to assess the in vitro effects of IFN-γ-pretreated microencapsulated (CpS)-hUCMS on T cells of pSS.Ten pSS patients and 10 healthy donors were selected. Peripheral blood mononuclear cells (PBMCs) were obtained from venous blood to establish co-cultures with CpS-hUCMS. Lymphocyte proliferation and phenotypic analysis was performed by flow cytometry and real-time PCR on IFN-γ-pretreated hUCMS was performed before PBMCs co-culture.We found that CpS-hUCMS suppress pSS T cell proliferation and restore the Treg/Th17 ratio, thereby possibly positively impacting the pSS disease process.We have developed a new biohybrid drug delivery system that now waits for clinical application in autoimmune diseases, including pSS. |
| تدمد: | 1462-0332 1462-0324 |
| DOI: | 10.1093/rheumatology/keu292 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ff076e073cb9d512ee136947e9be37c https://doi.org/10.1093/rheumatology/keu292 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4ff076e073cb9d512ee136947e9be37c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14620332 14620324 |
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| DOI: | 10.1093/rheumatology/keu292 |