Inhibition of zymosan-induced cytokine and chemokine expression in human corneal fibroblasts by triptolide
| العنوان: | Inhibition of zymosan-induced cytokine and chemokine expression in human corneal fibroblasts by triptolide |
|---|---|
| المؤلفون: | Ye Liu, Jing Li, Hui Jia, Ping Wang, Yang Liu |
| المصدر: | International Journal of Ophthalmology, Vol 9, Iss 1, Pp 9-14 (2016) |
| بيانات النشر: | Press of International Journal of Ophthalmology (IJO PRESS), 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Chemokine, medicine.medical_treatment, Inflammation, zymosan, Proinflammatory cytokine, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, lcsh:Ophthalmology, medicine, CXCL10, CCL13, biology, business.industry, Zymosan, Triptolide, Ophthalmology, Cytokine, Basic Research, chemistry, inflammation, lcsh:RE1-994, 030220 oncology & carcinogenesis, triptolide, Immunology, 030221 ophthalmology & optometry, biology.protein, Cancer research, fungal keratitis, medicine.symptom, corneal fibroblast, business |
| الوصف: | AIM: To investigate the effects of triptolide on proinflammatory cytokine and chemokine expression induced by the fungal component zymosan in cultured human corneal fibroblasts (HCFs). METHODS: HCFs were cultured in the absence or presence of zymosan or triptolide. The release of interleukin (IL)-6, IL-8, and monocyte chemoattractant protein-1 (MCP-1) into culture supernatants was measured with enzyme-linked immunosorbent assays. The cellular abundance of the mRNAs for these proteins was determined by reverse transcription and real-time polymerase chain reaction analysis. The phosphorylation of mitogen-activated protein kinases (MAPKs) and the endogenous nuclear factor-κB (NF-κB) inhibitor IκB-α was examined by immunoblot analysis. The release of lactate dehydrogenase (LDH) activity from HCFs was measured with a colorimetric assay. RESULTS: Triptolide inhibited the zymosan-induced release of IL-6, IL-8, and MCP-1 from HCFs in a concentration- and time-dependent manner. It also inhibited the zymosan-induced up-regulation of IL-6, IL-8, and MCP-1 mRNA abundance in these cells. Furthermore, triptolide attenuated zymosan-induced phosphorylation of the MAPKs extracellular signal-regulated kinase (ERK), c-Jun NH2-terminal kinase (JNK), and p38 as well as the phosphorylation and degradation of IκB-α. Triptolide did not exhibit cytotoxicity for HCFs. CONCLUSION: Triptolide inhibited proinflammatory cytokine and chemokine production by HCFs exposed to zymosan, with this action likely being mediated by suppression of MAPK and NF-κB signaling pathways. This compound might thus be expected to limit the infiltration of inflammatory cells into the cornea associated with fungal infection. |
| اللغة: | English |
| تدمد: | 2227-4898 2222-3959 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4fd7338fc7dd146678fe31baad84fbc5 https://doaj.org/article/1c2ce609391948c4b294bbaa36a7962f |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4fd7338fc7dd146678fe31baad84fbc5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22274898 22223959 |
|---|