Why mRNA-ionizable LNPs formulations are so short-lived: causes and way-out
| العنوان: | Why mRNA-ionizable LNPs formulations are so short-lived: causes and way-out |
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| المؤلفون: | Anindita, De, Young Tag, Ko |
| المصدر: | Expert Opinion on Drug Delivery. 20:175-187 |
| بيانات النشر: | Informa UK Limited, 2023. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Pharmaceutical Science |
| الوصف: | Messenger ribonucleic acid (mRNA) and small interfering RNA (siRNA) are biological molecules that can be heated, frozen, lyophilized, precipitated, or re-suspended without degradation. Currently, ionizable lipid nanoparticles (LNPs) are a promising approach for mRNA therapy. However, the long-term shelf-life stability of mRNA-ionizable LNPs is one of the open questions about their use and safety. At an acidic pH, ionizable lipids shield anionic mRNA. However, the stability of mRNA under storage conditions remains a mystery. Moreover, ionizable LNPs excipients also cause instability during long-term storage.This paper aims to illustrate why mRNA-ionizable LNPs have such a limited storage half-life. For the first time, we compile the tentative reasons for the short half-life and ultra-cold storage of mRNA-LNPs in the context of formulation excipients. The article also provided possible ways of prolonging the lifespan of mRNA-ionizable LNPs during long storage.mRNA-ionizable LNPs are the future of genetic medicine. Current limitations of the formulation can be overcome by an advanced drying process or a whole new hybrid formulation strategy to extend the shelf life of mRNA-ionizable LNPs. A breakthrough technology may open up new research directions for producing thermostable and safe mRNA-ionizable LNPs at room temperature. |
| تدمد: | 1744-7593 1742-5247 |
| DOI: | 10.1080/17425247.2023.2162876 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f9f788d084000159325940552b1bb2e https://doi.org/10.1080/17425247.2023.2162876 |
| رقم الانضمام: | edsair.doi.dedup.....4f9f788d084000159325940552b1bb2e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17447593 17425247 |
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| DOI: | 10.1080/17425247.2023.2162876 |