Protein kinase D1 overexpression potentiates epidermal growth factor signaling pathway in MCF-7 cells
| العنوان: | Protein kinase D1 overexpression potentiates epidermal growth factor signaling pathway in MCF-7 cells |
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| المؤلفون: | Christine Legay, Sophie Doublier, Sylvie Babajko, Jean-Marc Ricort |
| المصدر: | Molecular Biology Reports. 50:3641-3651 |
| بيانات النشر: | Springer Science and Business Media LLC, 2023. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Genetics, General Medicine, Molecular Biology |
| الوصف: | I. Background: Protein kinase D1, PKD1, is a serine-threonine kinase implicated in cell proliferation, migration, invasion, and/or apoptosis and its activation by several growth factors sets this enzyme as a key regulator of tumorigenesis and tumor progression. Despite many studies, its role in the regulation of intracellular signaling pathways remains widely disparate and needs to be clarified.II. Methods and Results: By using human breast cancer cells MCF-7, overexpressing or not PKD1, we demonstrated that PKD1 expression level modulated the tumor growth-promoting epidermal growth factor (EGF) signaling pathway. We also showed that EGF acutely stimulated PKD1 phosphorylation with similar time courses both in control and PKD1-overexpressing cells. However, PKD1 overexpression specifically and markedly increased EGF-induced phosphorylation of Akt (onto T308 and S473 residues) and extracellular-regulated protein kinase (ERK1/2). Finally, pharmacological inhibition of PKD1 activity or lowering its expression level using specific siRNAs drastically reduced EGF-stimulated Akt and ERK phosphorylation in PKD1overexpressing cells, but not in control cells.III. Conclusions: Overall, these results identified the level of PKD1 expression as a key determinant in the regulation of the EGF signaling pathway highlighting its crucial role in a tumorigenic setting. |
| تدمد: | 1573-4978 0301-4851 |
| DOI: | 10.1007/s11033-023-08300-z |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4e2ded27236ac4fc926f6907b1bd1238 https://doi.org/10.1007/s11033-023-08300-z |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4e2ded27236ac4fc926f6907b1bd1238 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15734978 03014851 |
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| DOI: | 10.1007/s11033-023-08300-z |