Isolation and Characterization of the HC8 Subunit Gene of the Human Proteasome

التفاصيل البيبلوغرافية
العنوان: Isolation and Characterization of the HC8 Subunit Gene of the Human Proteasome
المؤلفون: N.E. Forsberg, Katsuzumi Okumura, C. Noda, M. Nogami, N. Ishida, Keiji Tanaka, H. Akioka, Hiroshi Taguchi
المصدر: Biochemical and Biophysical Research Communications. 207:318-323
بيانات النشر: Elsevier BV, 1995.
سنة النشر: 1995
مصطلحات موضوعية: Chloramphenicol O-Acetyltransferase, Proteasome Endopeptidase Complex, Macromolecular Substances, Recombinant Fusion Proteins, Protein subunit, Molecular Sequence Data, Restriction Mapping, Biophysics, CAAT box, Regulatory Sequences, Nucleic Acid, Biology, Transfection, Polymerase Chain Reaction, Biochemistry, Chloramphenicol acetyltransferase, Multienzyme Complexes, Gene expression, Gene cluster, Animals, Humans, Cloning, Molecular, Promoter Regions, Genetic, Molecular Biology, Gene, In Situ Hybridization, Fluorescence, Chromosomes, Human, Pair 14, Genetics, Genomic Library, Base Sequence, Chromosome Mapping, Chromosome, Hominidae, Cell Biology, TATA Box, Molecular biology, Cysteine Endopeptidases, Gene Expression Regulation, Proteasome, Protein Biosynthesis, HeLa Cells
الوصف: For study of the molecular basis of regulation of proteasome gene expression, we isolated the gene encoding the alpha-type HC8 subunit of the human proteasome. About 2.3 kb of the 5' flanking region of this gene was tested for promoter function by chloramphenicol acetyltransferase assay. This analysis revealed that CAAT and TATA boxes, but not a GC box, are essential for its promoter activity. These results differed from previous findings that the genes for the alpha-type HC3 and beta-type HC5 subunits of the human proteasome have a TATA-less promoter and that two or three GC boxes function as the promoter sequences (Tamura, T. et al. (1994) J. Mol. Biol. 244, 1117-1124). We mapped the HC8 gene at q23 on human chromosome 14, which differs from the chromosomal locations of nine other proteasomal subunit genes mapped so far.
تدمد: 0006-291X
DOI: 10.1006/bbrc.1995.1190
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4da2787914f0b8aac9370285babe414b
https://doi.org/10.1006/bbrc.1995.1190
Rights: CLOSED
رقم الانضمام: edsair.doi.dedup.....4da2787914f0b8aac9370285babe414b
قاعدة البيانات: OpenAIRE
الوصف
تدمد:0006291X
DOI:10.1006/bbrc.1995.1190