Trichinella spiralis - New method for sample preparation and objective detection of specific antigens using a chemiluminescence immunoassay
| العنوان: | Trichinella spiralis - New method for sample preparation and objective detection of specific antigens using a chemiluminescence immunoassay |
|---|---|
| المؤلفون: | Caroline Pagneux, Monika Mackiewicz, Catherine Bardot, Jana Braasch, Stefanie Ostermann, Erik Lattwein, Viola Borchardt-Lohölter |
| المصدر: | Veterinary Parasitology: X Veterinary Parasitology: X, Vol 4, Iss, Pp 100033-(2020) |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Antigen detection, Muscle tissue, Chemiluminescence, Trichinella spiralis, Trichinella, digestive system, Article, Microbiology, Antigen, parasitic diseases, medicine, ComputingMethodologies_COMPUTERGRAPHICS, Immunoassay, Detection limit, Microscopy, Pig, lcsh:Veterinary medicine, Surveillance, General Veterinary, biology, medicine.diagnostic_test, fungi, General Medicine, Gold standard (test), Muscle larvae, biology.organism_classification, medicine.anatomical_structure, Meat inspection, lcsh:SF600-1100, Digestion, Parasitology |
| الوصف: | Graphical abstract Highlights • Introduction of a new sample preparation method involving shredding of pork. • Automated detection of specific T. spiralis antigens via chemiluminescence. • Trichinella ChLIA detects antigens of 1 larva in 100 g infected pork (0.01 lpg). • Objective evaluation via Trichinella ChLIA allows reliable meat inspection. • Meat inspection is faster with the new method than with the gold standard. The parasitic roundworm Trichinella spiralis is most commonly transmitted to humans through consumption of raw or undercooked meat of infected pigs or game. To prevent human infection, slaughterhouses perform meat safety surveillance using the gold standard “Magnetic Stirrer Method”. We introduce a fast and objective method using automated detection of specific Trichinella spiralis antigens by a newly developed immunoassay based on chemiluminescence (ChLIA). Panel A comprised muscle tissue samples from non-infected pigs (n = 37). Panel B comprised muscle tissue samples from non-infected pigs spiked with different amounts of Trichinella larvae without collagen capsules (n = 56). Panel C contained muscle tissue samples from experimentally infected pigs including Trichinella larvae encapsulated in collagen (n = 32). Each sample was shredded with PBS buffer in a knife mill, destroying Trichinella larvae. Following centrifugation, the supernatant (muscle tissue extract containing released excretory and secretory Trichinella spiralis antigens) was used for Trichinella-specific antigen detection by the new Trichinella ChLIA. The overall accuracy of the Trichinella ChLIA was 97.6 %. The specificity of the Trichinella ChLIA was 100 % (panel A). The sensitivity in samples from experimentally infected pigs was 100 % representing a detection limit of 0.01 larvae per gram. Cross-reactivity with parasites other than Trichinella spp. was not observed. This new meat inspection method for the detection of Trichinella spiralis antigens presents high specificity and high sensitivity, especially in truly infected samples. In contrast to the gold standard, this new approach to meat safety surveillance does not require longsome digestion or microscopy by trained personnel. |
| تدمد: | 1873-2550 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4d9a6ed7672753bdc07231f79df79fdd https://pubmed.ncbi.nlm.nih.gov/34392952 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4d9a6ed7672753bdc07231f79df79fdd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18732550 |
|---|