Evaluation of antiplasmodial activity and cytotoxicity assays of amino acids functionalized magnetite nanoparticles: Hyperthermia and flow cytometry applications
| العنوان: | Evaluation of antiplasmodial activity and cytotoxicity assays of amino acids functionalized magnetite nanoparticles: Hyperthermia and flow cytometry applications |
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| المؤلفون: | Ricardo Costa de Santana, Yury Oliveira Chaves, Marco A. Morales, Miguel Jafelicci, Caio Carvalho dos Santos, João Victor Brandt, Anna C. Bakenecker, Luis André Morais Mariúba, Bruno E. Amantéa, Kerstin Lüdtke-Buzug, Anselmo Fortunato Ruiz Rodriguez, Rodrigo Fernando Costa Marques |
| المصدر: | Materials scienceengineering. C, Materials for biological applications. 125 |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Materials science, Nanoparticle, Bioengineering, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Biomaterials, Antimalarials, Magnetic particle imaging, Dynamic light scattering, Spectroscopy, Fourier Transform Infrared, Zeta potential, Humans, Hyperthermia, Fourier transform infrared spectroscopy, Amino Acids, Magnetite Nanoparticles, 021001 nanoscience & nanotechnology, Flow Cytometry, Antiparasitic agent, 0104 chemical sciences, Magnetic hyperthermia, Mechanics of Materials, Magnetic nanoparticles, 0210 nano-technology, Nuclear chemistry |
| الوصف: | We report the synthesis of magnetite nanoparticles (MNP) and their functionalization with glycine (MNPGly), β-alanine (MNPAla), L-phenylalanine (MNPPhAla), D-(−)-α-phenylglycine (MNPPhGly) amino acids. The functionalized nanoparticles were characterized by Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), electron paramagnetic resonance (EPR), vibrating sample magnetometry (VSM), Mossbauer spectroscopy (MS), magnetic hyperthermia (MH), dynamic light scattering and zeta potential. The functionalized nanoparticles had isoelectric points (IEP) at pH ≃ 4.4, 5.8, 5.9 and 6.8 for samples MNPGly, MNPAla, MNPPhGly and MNPPhAla, respectively, while pure magnetite had an IEP at pH 5.6. In the MH experiments, the samples showed specific absorption rate (SAR) of 64, 71, 74, 81 and 66 W/g for MNP, MNPGly, MNPAla, MNPPhGly, and MNPPhAla, respectively. We used a flow cytometric technique to determine the cellular magnetic nanoparticles plus amino acids content. Magnetic fractionation and characterization of Resovist® magnetic nanoparticles were performed for applications in magnetic particle imaging (MPI). We have also studied the antiproliferative and antiparasitic effects of functionalized MNPs. Overall, the data showed that the functionalized nanoparticles have great potential for using as environmental, antitumor, antiparasitic agents and clinical applications. |
| تدمد: | 1873-0191 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4d20637f19e4b6ae7afbc3c0daa0339a https://pubmed.ncbi.nlm.nih.gov/33965107 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....4d20637f19e4b6ae7afbc3c0daa0339a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18730191 |
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