Cannabinoid receptor activation inhibits cell cycle progression bymodulating 14-3-3 beta
| العنوان: | Cannabinoid receptor activation inhibits cell cycle progression bymodulating 14-3-3 beta |
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| المؤلفون: | Hye-Won Jung, Inae Park, Sungho Ghil |
| المصدر: | CELLULAR & MOLECULAR BIOLOGY LETTERS(19): 3 Cellular & Molecular Biology Letters |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | G2 Phase, Cannabinoid receptor, Short Communication, medicine.medical_treatment, Immunoblotting, Population, Cdc2, Cyclin B, Biochemistry, HeLa, Mice, Receptor, Cannabinoid, CB1, Two-Hybrid System Techniques, medicine, Animals, Humans, Wee1, Phosphorylation, education, Molecular Biology, Cyclin-dependent kinase 1, education.field_of_study, biology, Cannabinoids, Cdc25B, G(2)/M phase, GIPs, Yeast-two hybrid, Cell Cycle, HEK 293 cells, G2/M phase, Cell Biology, Cell cycle, Flow Cytometry, Cell biology, G2 Phase Cell Cycle Checkpoints, HEK293 Cells, 14-3-3 Proteins, biology.protein, Cannabinoid, Cell Division, Intracellular, HeLa Cells, Protein Binding, Signal Transduction |
| الوصف: | Cannabinoids display various pharmacological activities, including tumor regression, anti-inflammatory and neuroprotective effects. To investigate the molecular mechanisms underlying the pharmacological effects of cannabinoids, we used a yeast two-hybrid system to screen a mouse brain cDNA library for proteins interacting with type 1 cannabinoid receptor (CB1R). Using the intracellular loop 3 of CB1R as bait, we identified 14-3-3β as an interacting partner of CB1R and confirmed their interaction using affinity-binding assays. 14-3-3β has been reported to induce a cell cycle delay at the G2/M phase. We tested the effects of cannabinoids on cell cycle progression in HeLa cells synchronized using a double-thymidine block-and-release protocol and found an increase in the population of G2/M phase cells. We further found that CB1R activation augmented the interaction of 14-3-3β with Wee1 and Cdc25B, and promoted phosphorylation of Cdc2 at Tyr-15. These results suggest that cannabinoids induce cell cycle delay at the G2/M phase by activating 14-3-3β. |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4c38f4908f52eb1967ad1a6c545ff109 http://open-repository.kisti.re.kr/cube/handle/open_repository/475675.do |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4c38f4908f52eb1967ad1a6c545ff109 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |