Regeneration of a Bioengineered Thyroid Using Decellularized Thyroid Matrix
| العنوان: | Regeneration of a Bioengineered Thyroid Using Decellularized Thyroid Matrix |
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| المؤلفون: | Hui Li, Ying-ying Wang, Yan Wang, Xin-Hui Zhou, Xiong-Fei Yu, Ye-Hui Du, Yi-Jun Wu, Li-Xian Zhu, Yi-Bin Shen, Xue-Yu Zhou, Feng Zhu, Yun Fang, Jun Pan |
| المصدر: | Thyroid. 29:142-152 |
| بيانات النشر: | Mary Ann Liebert Inc, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | endocrine system, endocrine system diseases, Endocrinology, Diabetes and Metabolism, Thyroid Gland, 030209 endocrinology & metabolism, Matrix (biology), Regenerative medicine, 03 medical and health sciences, 0302 clinical medicine, Endocrinology, Hypothyroidism, medicine, Animals, Regeneration, Decellularization, Tissue Engineering, Chemistry, Regeneration (biology), Thyroid, Extracellular Matrix, Rats, Cell biology, Extracellular matrix scaffold, medicine.anatomical_structure, Thyroid Epithelial Cells, 030220 oncology & carcinogenesis, hormones, hormone substitutes, and hormone antagonists, Hormone |
| الوصف: | Hypothyroidism is a common hormone deficiency condition. Regenerative medicine approaches, such as a bioengineered thyroid, have been proposed as potential therapeutic alternatives for patients with hypothyroidism. This study demonstrates a novel approach to generate thyroid grafts using decellularized rat thyroid matrix.Isolated rat thyroid glands were perfused with 1% sodium dodecyl sulfate to generate a decellularized thyroid scaffold. The rat thyroid scaffold was then recellularized with rat thyroid cell line to reconstruct the thyroid by perfusion seeding technique. As a pilot study, the decellularized rat thyroid scaffold was perfused with human-derived thyrocytes and parathyroid cells.The decellularization process retained the intricate three-dimensional microarchitecture with a perfusable vascular network and native extracellular matrix components, allowing efficient reseeding of the thyroid matrix with the FRTL-5 rat thyroid cell line generating three-dimensional follicular structures in vitro. In addition, the recellularized thyroid showed successful cellular engraftment and thyroid-specific function, including synthesis of thyroglobulin and thyroid peroxidase. Moreover, the decellularized rat thyroid scaffold could further be recellularized with human-derived thyroid cells and parathyroid cells to reconstruct a humanized bioartificial endocrine organ, which maintained expression of critical genes such as thyroglobulin, thyroid peroxidase, and parathyroid hormone.These findings demonstrate the utility of a decellularized thyroid extracellular matrix scaffold system for the development of functional, bioengineered thyroid tissue, which could potentially be used to treat hypothyroidism. |
| تدمد: | 1557-9077 1050-7256 |
| DOI: | 10.1089/thy.2018.0068 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4af0c80578e3d1a8a29f7dc31a5f1559 https://doi.org/10.1089/thy.2018.0068 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....4af0c80578e3d1a8a29f7dc31a5f1559 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15579077 10507256 |
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| DOI: | 10.1089/thy.2018.0068 |