Optimization of the spontaneous tail coiling test for fast assessment of neurotoxic effects in the zebrafish embryo using an automated workflow in KNIME®
| العنوان: | Optimization of the spontaneous tail coiling test for fast assessment of neurotoxic effects in the zebrafish embryo using an automated workflow in KNIME® |
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| المؤلفون: | Afolarin O. Ogungbemi, Eberhard Küster, Stefan Scholz, Riccardo Massei, Elisabet Teixidó |
| المصدر: | Dipòsit Digital de la UB Universidad de Barcelona Neurotoxicology and teratology |
| بيانات النشر: | Elsevier, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Hiperactivitat, Neurotoxicology, Diazinon, Embryo, Nonmammalian, Alternatives to animal testing, 010501 environmental sciences, Pharmacology, Toxicology, 01 natural sciences, Paraoxon, Workflow, Nicotine, 03 medical and health sciences, Cellular and Molecular Neuroscience, chemistry.chemical_compound, 0302 clinical medicine, Developmental Neuroscience, medicine, Animals, Prospective Studies, Mode of action, Zebrafish, 0105 earth and related environmental sciences, Neurotoxicologia, Pharmacology. Therapy, Enzyme inhibitors, Carbamazepine, Embryo, Mammalian, Acetilcolinesterasa, Hyperactivity, chemistry, Inhibidors enzimàtics, Chlorpyrifos, Zebrafish embryo, Acetylcholinesterase, Neurotoxicity Syndromes, Human medicine, Diazepam, 030217 neurology & neurosurgery, Water Pollutants, Chemical, medicine.drug |
| الوصف: | Neuroactive chemicals are frequently detected in the environment. At sufficiently high concentrations or within mixtures, they could provoke neurotoxic effects and neurological diseases to organisms and humans. Fast identification of such neuroactive compounds in the environment could help in hazard assessment and risk mitigation. Behavior change is considered as an important endpoint and might be directly or indirectly connected to a neuroactive mode of action. For a fast evaluation of environmental samples and pure substances, we optimized the measurement of a behavioral endpoint in zebrafish embryos - the spontaneous tail coiling (STC). Evaluation of results is automated via the use of a workflow established with the KNIME (R) software. Analysis duration and developmental stage were optimized to 1 min and 25 +/- 1 hpf respectively during measurement. Exposing the embryos in a group of 10 or 20 and acclimatizing for 30 min at room temperature proved to be reliable. The optimized method was used to investigate neurotoxic effects of 18 substances with different modes of action (MoA). The STC WA accurately detected the effect of 8 out of 11 neuroactive substances (chlorpyrifos, chlorpyrifos-oxon, diazinon, paraoxon-methyl, abamectin, carbamazepine, propafenone and diazepam). Aldicarb and nicotine showed subtle effects which were considered to be conditional and imidacloprid showed no effect. For substances with unknown neuroactive MoA, 3 substances did not provoke any effect on the STC (pyraclostrobin, diuron and daunorubicin-hydrochloride) while 4 other substances provoked an increased STC (hexaconazole, aniline, dimethyl-sulfoxide and 3,4-dichloroaniline). Such unexpected effects indicate possible neuroactive side effects or unknown mechanisms of action that impact on the STC. In conclusion, the optimized STC parameters and the automated analysis in KNIME (R) indicate opportunities for the harmonization of the STC WA and further development for prospective and diagnostic testing. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0892-0362 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4a503435e5df277b36eb717f8539aa6d http://hdl.handle.net/2445/175964 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4a503435e5df277b36eb717f8539aa6d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 08920362 |
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