Assessment of Effects of Si-Ca-P Biphasic Ceramic on the Osteogenic Differentiation of a Population of Multipotent Adult Human Stem Cells
| العنوان: | Assessment of Effects of Si-Ca-P Biphasic Ceramic on the Osteogenic Differentiation of a Population of Multipotent Adult Human Stem Cells |
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| المؤلفون: | Ruben Rabadan-Ros, Piedad N. De Aza, A. Murciano, Patricia Ros-Tárraga, Luis Meseguer-Olmo |
| المصدر: | Materials, Vol 9, Iss 12, p 969 (2016) Materials; Volume 9; Issue 12; Pages: 969 Materials |
| بيانات النشر: | MDPI AG, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | bioceramic, calcium silicophosphate, eutectoid, adult human mesenchymal stem cells, cell culture, osteogenic differentiation, Materials science, Population, 02 engineering and technology, Bioceramic, 010402 general chemistry, 01 natural sciences, lcsh:Technology, Article, Extracellular matrix, medicine, General Materials Science, education, lcsh:Microscopy, lcsh:QC120-168.85, education.field_of_study, lcsh:QH201-278.5, lcsh:T, Regeneration (biology), Mesenchymal stem cell, Osteoblast, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Cell biology, medicine.anatomical_structure, Cell culture, lcsh:TA1-2040, lcsh:Descriptive and experimental mechanics, lcsh:Electrical engineering. Electronics. Nuclear engineering, Stem cell, 0210 nano-technology, lcsh:Engineering (General). Civil engineering (General), lcsh:TK1-9971, Biomedical engineering |
| الوصف: | A new type of bioceramic with osteogenic properties, suitable for hard tissue regeneration, was synthesised. The ceramic was designed and obtained in the Nurse's A-phase-silicocarnotite subsystem. The selected composition was that corresponding to the eutectoid 28.39 wt % Nurse's A-phase-71.61 wt % silicocarnotite invariant point. We report the effect of Nurse's A-phase-silicocarnotite ceramic on the capacity of multipotent adult human mesenchymal stem cells (ahMSCs) cultured under experimental conditions, known to adhere, proliferate and differentiate into osteoblast lineage cells. The results at long-term culture (28 days) on the material confirmed that the undifferentiated ahMSCs cultured and in contact with the material surface adhered, spread, proliferated, and produced a mineralised extracellular matrix on the studied ceramic, and finally acquired an osteoblastic phenotype. These findings indicate that it underwent an osteoblast differentiation process. All these findings were more significant than when cells were grown on plastic, in the presence and absence of this osteogenic supplement, and were more evident when this supplement was present in the growth medium (GM). The ceramic evaluated herein was bioactive, cytocompatible and capable of promoting the proliferation and differentiation of undifferentiated ahMSCs into osteoblasts, which may be important for bone integration into the clinical setting. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1996-1944 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::49ecf3cd7183d27f85540394fcffbf65 http://www.mdpi.com/1996-1944/9/12/969 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....49ecf3cd7183d27f85540394fcffbf65 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19961944 |
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