Specificity of a novel TaqMan PCR method for detection of poultry DNA
| العنوان: | Specificity of a novel TaqMan PCR method for detection of poultry DNA |
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| المؤلفون: | Ingrid M. J. Scholtens, Theo W. Prins, Leo W.D. van Raamsdonk |
| المصدر: | Food Control 73 (2017) Food Control, 73, 532-539 |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Veterinary medicine, Novel Foods & Agrochains, BU Toxicologie, Animal feed, Bovine spongiform encephalopathy, Species-to-species ban, Model system, Novel Foods & Agroketens, 01 natural sciences, Poultry, chemistry.chemical_compound, 0404 agricultural biotechnology, Goose, biology.animal, Processed Animal Protein (PAP), TaqMan, medicine, media_common.cataloged_instance, BU Toxicology, Novel Foods & Agrochains, European union, media_common, biology, BU Toxicology, 010401 analytical chemistry, Transmissible Spongiform Encephalopathy (TSE), 04 agricultural and veterinary sciences, medicine.disease, 040401 food science, Virology, 0104 chemical sciences, chemistry, BU Toxicologie, Novel Foods & Agroketens, Feed, Bovine Spongiform Encephalopathy (BSE), Pcr method, DNA, Food Science, Biotechnology |
| الوصف: | After the Bovine Spongiform Encephalopathy (BSE) crisis emerged in 1985/1986, all processed animal proteins (PAPs) were finally banned for use in animal feed in the European Union. To partially lift this feed ban, paths for re-introduction of PAPs from species other than ruminants e.g. pig and poultry, are described in the Transmissible Spongiform Encephalopathies (TSE) Roadmap 2. Cannibalism, however, is still not allowed. Specific detection methods for pig and poultry meal and PAPs are prerequisites for reintroduction of pig and poultry processed animal proteins into animal feed. Developing a sensitive PCR method that specifically detects the taxonomically diverse and therefore artificial group ‘poultry’ and that does not detect other birds at the same time is a challenge. Here, a novel TaqMan PCR method for poultry detection is presented. The specificity of the poultry method against target and non-target species has been extensively investigated. The efficiency, linearity and sensitivity was tested using dilution series of chicken, turkey, duck and goose DNA isolated from meat and autoclaved meat as a model system for PAPs. |
| وصف الملف: | application/pdf; application/octet-stream |
| اللغة: | English |
| تدمد: | 0956-7135 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::49489be4d9df17d778f3e311a2d2024f https://research.wur.nl/en/publications/specificity-of-a-novel-taqman-pcr-method-for-detection-of-poultry |
| Rights: | RESTRICTED |
| رقم الانضمام: | edsair.doi.dedup.....49489be4d9df17d778f3e311a2d2024f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 09567135 |
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