Stimulation of proximal small intestinal mucosal growth by luminal polyamines
| العنوان: | Stimulation of proximal small intestinal mucosal growth by luminal polyamines |
|---|---|
| المؤلفون: | Jian-Ying Wang, Leonard R. Johnson, Mary Jane Viar, S. A. Mccormack |
| المصدر: | American Journal of Physiology-Gastrointestinal and Liver Physiology. 261:G504-G511 |
| بيانات النشر: | American Physiological Society, 1991. |
| سنة النشر: | 1991 |
| مصطلحات موضوعية: | DNA Replication, Male, medicine.medical_specialty, Eflornithine, Duodenum, Spermidine, Physiology, Spermine, Biology, Ornithine Decarboxylase, Tritium, Cell Line, Ornithine decarboxylase, Jejunum, chemistry.chemical_compound, Physiology (medical), Internal medicine, medicine, Animals, Intestinal Mucosa, Hepatology, DNA synthesis, Gastroenterology, Proteins, Rats, Inbred Strains, DNA, Small intestine, Rats, Kinetics, medicine.anatomical_structure, Endocrinology, chemistry, Putrescine, RNA, Polyamine, Thymidine |
| الوصف: | The purpose of this study was to determine whether luminal polyamines stimulate intestinal mucosal growth in vivo. Rats received 2% alpha-difluoromethylornithine (DFMO) added to their drinking water throughout the experiment. The polyamines spermidine and spermine (3 mg each/100 g body wt) were given intragastrically in combined doses once at 9:30 A.M. and again at 5:30 P.M. Duodenal and jejunal mucosal ornithine decarboxylase (ODC) activity in the DFMO-treated rats was inhibited significantly for the duration of the study. DFMO also markedly decreased the rate of [3H]thymidine incorporation into DNA of duodenal and jejunal mucosa. The decrease in [3H]thymidine incorporation was significant 4 days and maximal 6 and 8 days after beginning treatment with DFMO. Decreased ODC activity and DNA synthesis were paralleled by decreases in total mucosal DNA, RNA, and protein content. Administration of the polyamines significantly reversed the effects of DFMO except the inhibition of ODC. In fact, there were no significant differences in mucosal growth parameters between the controls (without DFMO) and those treated with DFMO plus polyamines. Oral administration of spermidine and spermine at a dose of 4.5 mg each/100 g body wt for 6 days to rats not treated with DFMO increased the normal rate of mucosal growth in the duodenum and jejunum as well. Polyamine accumulation in IEC-6 cells was measured to determine whether it was altered by DFMO. IEC-6 cells took up [3H]putrescine and [3H]spermidine from their surrounding environment and the uptake was stimulated by serum. DFMO (5 mM) totally inhibited the increase in ODC activity but had no effect on the cellular uptake of polyamines in the presence of putrescine.(ABSTRACT TRUNCATED AT 250 WORDS) |
| تدمد: | 1522-1547 0193-1857 |
| DOI: | 10.1152/ajpgi.1991.261.3.g504 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::48fa9aea725b04128082475f3a1e50f2 https://doi.org/10.1152/ajpgi.1991.261.3.g504 |
| رقم الانضمام: | edsair.doi.dedup.....48fa9aea725b04128082475f3a1e50f2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15221547 01931857 |
|---|---|
| DOI: | 10.1152/ajpgi.1991.261.3.g504 |