The intermediary metabolite pyruvate attenuates stunning and reduces infarct size in in vivo porcine myocardium
| العنوان: | The intermediary metabolite pyruvate attenuates stunning and reduces infarct size in in vivo porcine myocardium |
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| المؤلفون: | Yukihiro Yoshimura, Robert D. Lasley, Gentian Kristo, Rolf Bünger, Byron J. Keith, Jianli Niu, Robert M. Mentzer |
| المصدر: | American Journal of Physiology-Heart and Circulatory Physiology. 286:H517-H524 |
| بيانات النشر: | American Physiological Society, 2004. |
| سنة النشر: | 2004 |
| مصطلحات موضوعية: | Swine, Physiology, Partial Pressure, Metabolite, Myocardial Infarction, Myocardial Ischemia, In Vitro Techniques, Pharmacology, medicine.disease_cause, chemistry.chemical_compound, In vivo, Coronary Circulation, Physiology (medical), Animals, Medicine, cardiovascular diseases, Pyruvates, Myocardial Stunning, business.industry, Sodium, Stunning, Carbon Dioxide, Infarct size, In vitro, Intermediary metabolite, Bicarbonates, Disease Models, Animal, chemistry, Anesthesia, Circulatory system, Cardiology and Cardiovascular Medicine, business, Oxidative stress |
| الوصف: | The intermediary metabolite pyruvate has been shown to exert significant beneficial effects in in vitro models of myocardial oxidative stress and ischemia-reperfusion injury. However, there have been few reports of the ability of pyruvate to attenuate myocardial stunning or reduce infarct size in vivo. This study tested whether supraphysiological levels of pyruvate protect against reversible and irreversible in vivo myocardial ischemia-reperfusion injury. Anesthetized, open-chest pigs ( n = 7/group) underwent 15 min of left anterior descending coronary artery (LAD) occlusion and 3 h of reperfusion to induce stunning. Load-insensitive contractility measurements of regional preload recruitable stroke work (PRSW) and PRSW area (PRSWA) were generated. Vehicle or pyruvate (100 mg/kg iv bolus + 10 mg·kg–1·min–1 intra-atrial infusion) was administered during ischemia and for the first hour of reperfusion. In infarct studies, pigs ( n = 6/group) underwent 1 h of LAD ischemia and 3 h of reperfusion. Group I pigs received vehicle or pyruvate for 30 min before and throughout ischemia. In group II, the infusion was extended through 1 h of reperfusion. In the stunning protocol, pyruvate significantly improved the recovery of PRSWA at 1 h (50 ± 4% vs. 23 ± 3% in controls) and 3 h (69 ± 5% vs. 39 ± 3% in controls) reperfusion. Control pigs exhibited infarct sizes of 66 ± 1% of the area at risk. The pyruvate I protocol was associated with an infarct size of 49 ± 3% ( P < 0.05), whereas the pyruvate II protocol was associated with an infarct size of 30 ± 2% ( P < 0.05 vs. control and pyruvate I). These findings suggest that pyruvate attenuates stunning and decreases myocardial infarction in vivo in part by reduction of reperfusion injury. Metabolic interventions such as pyruvate should be considered when designing the optimal therapeutic strategies for limiting myocardial ischemia-reperfusion injury. |
| تدمد: | 1522-1539 0363-6135 |
| DOI: | 10.1152/ajpheart.00777.2003 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::485a3da9e08e62846e168eda6543e0bb https://doi.org/10.1152/ajpheart.00777.2003 |
| رقم الانضمام: | edsair.doi.dedup.....485a3da9e08e62846e168eda6543e0bb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15221539 03636135 |
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| DOI: | 10.1152/ajpheart.00777.2003 |