Overexpression of an AP2/ERF type transcription factor OsEREBP1 confers biotic and abiotic stress tolerance in rice
| العنوان: | Overexpression of an AP2/ERF type transcription factor OsEREBP1 confers biotic and abiotic stress tolerance in rice |
|---|---|
| المؤلفون: | Axel Mithöfer, Jyothilakshmi Vadassery, Lavanya Dampanaboina, Rajeshwari Ramanan, Saivishnupriya Kappara, V. Jisha |
| المصدر: | PLoS One PLoS ONE, Vol 10, Iss 6, p e0127831 (2015) PLoS ONE |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | lcsh:Medicine, Biology, Real-Time Polymerase Chain Reaction, chemistry.chemical_compound, Xanthomonas oryzae, Plant Growth Regulators, Gene Expression Regulation, Plant, Stress, Physiological, RNA, Messenger, Jasmonate, lcsh:Science, Abscisic acid, Transcription factor, Oligonucleotide Array Sequence Analysis, Plant Diseases, Plant Proteins, Genetics, Multidisciplinary, Reverse Transcriptase Polymerase Chain Reaction, Abiotic stress, Gene Expression Profiling, Jasmonic acid, fungi, lcsh:R, food and beverages, Oryza, Biotic stress, Plants, Genetically Modified, biology.organism_classification, Genetically modified rice, Droughts, Cell biology, chemistry, lcsh:Q, Biomarkers, Research Article, Transcription Factors |
| الوصف: | AP2/ERF-type transcription factors regulate important functions of plant growth and development as well as responses to environmental stimuli. A rice AP2/ERF transcription factor, OsEREBP1 is a downstream component of a signal transduction pathway in a specific interaction between rice (Oryza sativa) and its bacterial pathogen, Xoo (Xanthomonas oryzae pv. oryzae). Constitutive expression of OsEREBP1 in rice driven by maize ubiquitin promoter did not affect normal plant growth. Microarray analysis revealed that over expression of OsEREBP1 caused increased expression of lipid metabolism related genes such as lipase and chloroplastic lipoxygenase as well as several genes related to jasmonate and abscisic acid biosynthesis. PR genes, transcription regulators and Aldhs (alcohol dehydrogenases) implicated in abiotic stress and submergence tolerance were also upregulated in transgenic plants. Transgenic plants showed increase in endogenous levels of α-linolenate, several jasmonate derivatives and abscisic acid but not salicylic acid. Soluble modified GFP (SmGFP)-tagged OsEREBP1 was localized to plastid nucleoids. Comparative analysis of non-transgenic and OsEREBP1 overexpressing genotypes revealed that OsEREBP1 attenuates disease caused by Xoo and confers drought and submergence tolerance in transgenic rice. Our results suggest that constitutive expression of OsEREBP1 activates the jasmonate and abscisic acid signalling pathways thereby priming the rice plants for enhanced survival under abiotic or biotic stress conditions. OsEREBP1 is thus, a good candidate gene for engineering plants for multiple stress tolerance. |
| وصف الملف: | application/pdf; application/zip |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::472ac1d3ef1ebc4cef9526f952d87555 https://hdl.handle.net/11858/00-001M-0000-0027-7F88-111858/00-001M-0000-0026-C824-B21.11116/0000-0002-DB6E-D |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....472ac1d3ef1ebc4cef9526f952d87555 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |