Identification of a dioxin-responsive oxylipin signature in roots of date palm: involvement of a 9-hydroperoxide fatty acid reductase, caleosin/peroxygenase PdPXG2
| العنوان: | Identification of a dioxin-responsive oxylipin signature in roots of date palm: involvement of a 9-hydroperoxide fatty acid reductase, caleosin/peroxygenase PdPXG2 |
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| المؤلفون: | Ibrahem Almousally, Abdulsamie Hanano, Denis J. Murphy, Mouhnad Shaban |
| المصدر: | Scientific Reports, Vol 8, Iss 1, Pp 1-19 (2018) Scientific Reports |
| بيانات النشر: | Springer Science and Business Media LLC, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Models, Molecular, 0106 biological sciences, 0301 basic medicine, Gene isoform, lcsh:Medicine, Dioxins, Plant Roots, 01 natural sciences, Hazardous air pollutants, Article, Mixed Function Oxygenases, 03 medical and health sciences, chemistry.chemical_compound, Gene Expression Regulation, Plant, Suberin, heterocyclic compounds, Oxylipins, lcsh:Science, Plant Proteins, Regulation of gene expression, Multidisciplinary, Fatty acid reductase, Chemistry, lcsh:R, Phoeniceae, food and beverages, Oxylipin, Aldehyde Oxidoreductases, In vitro, 030104 developmental biology, Biochemistry, Environmental Pollutants, lcsh:Q, Xenobiotic, 010606 plant biology & botany |
| الوصف: | Dioxins are highly hazardous pollutants that have well characterized impacts on both animal and human health. However, the biological effects of dioxins on plants have yet to be described in detail. Here we describe a dioxin-inducible caleosin/peroxygenase isoform, PdPXG2, that is mainly expressed in the apical zone of date palm roots and specifically reduces 9-hydroperoxide fatty acids. A characteristic spectrum of 18 dioxin-responsive oxylipin (DROXYL) congeners was also detected in date palm roots after exposure to dioxin. Of particular interest, six oxylipins, mostly hydroxy fatty acids, were exclusively formed in response to TCDD. The DROXYL signature was evaluated in planta and validated in vitro using a specific inhibitor of PdPXG2 in a root-protoplast system. Comparative analysis of root suberin showed that levels of certain monomers, especially the mono-epoxides and tri-hydroxides of C16:3 and C18:3, were significantly increased after exposure to TCDD. Specific inhibition of PdPXG2 activity revealed a positive linear relationship between deposition of suberin in roots and their permeability to TCDD. The results highlight the involvement of this peroxygenase in the plant response to dioxin and suggest the use of dioxin-responsive oxylipin signatures as biomarkers for plant exposure to this important class of xenobiotic contaminants. |
| تدمد: | 2045-2322 |
| DOI: | 10.1038/s41598-018-31342-4 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::46510d129d68c642c032d1e9ad7fec52 https://doi.org/10.1038/s41598-018-31342-4 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....46510d129d68c642c032d1e9ad7fec52 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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| DOI: | 10.1038/s41598-018-31342-4 |