Proteomic analysis of hypoxia and non-hypoxia secretome mesenchymal stem-like cells from human breastmilk
| العنوان: | Proteomic analysis of hypoxia and non-hypoxia secretome mesenchymal stem-like cells from human breastmilk |
|---|---|
| المؤلفون: | Sri Lilidjanti Widjaja, Soetrisno, Indah Yulianto, Harsono Salimo |
| المصدر: | Saudi Journal of Biological Sciences, Vol 28, Iss 8, Pp 4399-4407 (2021) Saudi Journal of Biological Sciences |
| بيانات النشر: | Elsevier, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Angiogenesis, Cell, TGF-β, Transforming Growth Factor-Beta, 01 natural sciences, HIF-1α, Hypoxia Inducible Factor-1α, ATP, Adenosine Triphosphate, Biology (General), Hypoxia, Secretome, PBS, Phosphate-buffered Saline, biology, medicine.diagnostic_test, VEGF, Vascular Endothelial Growth Factor, IGF1, Insulin-like Growth Factor 1, LF, Lactoferrin, SMAD, Signals Mothers Against the Decapentaplegic, LC-MS, Liquid Chromatography-Mass Spectrometry, MSC, Mesenchymal Stem Cell, Cell biology, hBSC, medicine.anatomical_structure, Original Article, Stem cell, General Agricultural and Biological Sciences, MPS, Multi Proliferative Supplement, Mesenchymal stem-like cell, BMPR-II, Bone morphogenetic protein type II, FACS, Fluorescence-Activated Cell Sorting, EHD3, EH Domain-containing Protein 3, QH301-705.5, SMA, Smooth Muscle Actin, cDNA, complementary Deoxyribonucleic Acid, hBSC, Human Breastmilk Stem Cell, Flow cytometry, AFP, Alpha-Fetoprotein, 03 medical and health sciences, mRNA, messenger Ribonucleic Acid, medicine, CD90, BD, Becton Dickinson, CD44, Mesenchymal stem cell, MAPK, Mitogen-Activated Protein Kinase, LALBA, α-Lactalbumin, LC-MS, 030104 developmental biology, Cell culture, biology.protein, MPZL1, Myelin Protein Zero-like Protein 1, BSA, Bovine Serum Albumin, SDS, Sodium Dodecyl Sulfate, 010606 plant biology & botany, FBS, Fetal Bovine Serum |
| الوصف: | Introduction Breastmilk contains proteins and cells which have stem cell properties. The human breastmilk stem cell mimick mesenchymal stem cells and expresses pluripotency genes. The protein level of breastmilk is high in colostrum and gradually subsides in the first year of lactation. The mesenchymal stem cells from breastmilk can be an alternative source of stem cells that can potentially affect cardiovascular therapy. This study aimed to identify the proteomic analysis of secretome mesenchymal stem-like cells under hypoxia compared to non-hypoxia from human breastmilk stem cells. Material and methods The human breastmilk was collected from six healthy breastfeeding women and transported to the laboratory under aseptic conditions. The breastmilk cells were isolated then cultured. After 72 h, the human breastmilk stem cells reached confluence then cleaned up and isolated in serum-free media (spheroid) to allow serial passaging every 48 h. The acquisition stem cell was made with flow cytometry. The cells were divided into hBSC secretomes under hypoxia (A) and non-hypoxia (B) and analyzed for LC-MS to identify the peptide structure. Results The human breastmilk cells contained several mesenchymal stem-like cells in density 2.4 × 106 cell/mL for hypoxia and 2 × 106 cell/mL for non-hypoxia conditions. The human breastmilk stem cell surface markers derived from the third cell passage process were 93.77% for CD44, 98.69% for CD73, 88.45% for CD90, and 96.30% for CD105. The protein level of secretome mesenchymal stem -like cells under hypoxia was measured at 5.56 μg/mL and 4.28 μg/mL for non-hypoxia. The liquid chromatography-mass spectrometry analysis identified 130 and 59 peptides from hypoxia and non-hypoxia of the human breastmilk stem cell secretome sequentially. Some important proteomics structures were found in the hypoxic human breastmilk stem cell secretome, such as transforming growth factor-β, VE-cadherin, and caspase. Conclusion The human breastmilk cells contain mesenchymal stem-like cells and a high concentration of CD44, CD73, CD90, and CD105 as surface markers at third passage culture. The hypoxic hBSC secretome produces a higher protein level compare to non-hypoxia. The transforming growth factor -β was found in the hypoxic hBSC secretome as a modulator of VEGF-mediated angiogenesis. |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4645402521682a63a0ae41a6d9b587ca http://www.sciencedirect.com/science/article/pii/S1319562X21002928 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4645402521682a63a0ae41a6d9b587ca |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |