Effect of different semen extenders for the storage of chilled sperm in Tigrina ( Leopardus tigrinus )
| العنوان: | Effect of different semen extenders for the storage of chilled sperm in Tigrina ( Leopardus tigrinus ) |
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| المؤلفون: | Marcelo Alcindo de Barros Vaz Guimarães, Valquíria Hyppólito Barnabé, Marcilio Nichi, Carmen Neusa Martins Cortada, D. S. R. Angrimani, João Diego de Agostini Losano, S.H.R. Correa, Ricardo Pimenta Bertolla, P. M. H Barros |
| المصدر: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| بيانات النشر: | Elsevier BV, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Male, Felidae, medicine.medical_treatment, Semen, Biology, BIOTECNOLOGIA DA REPRODUÇÃO, Cryopreservation, law.invention, Andrology, 03 medical and health sciences, Cryoprotective Agents, 0302 clinical medicine, Human fertilization, Food Animals, Refrigeration, law, medicine, Animals, Small Animals, Sperm motility, 030219 obstetrics & reproductive medicine, Equine, Artificial insemination, Extender, 0402 animal and dairy science, 04 agricultural and veterinary sciences, Anatomy, Egg Yolk, Glutathione, Spermatozoa, 040201 dairy & animal science, Semen cryopreservation, Sperm, Sperm Motility, Animal Science and Zoology, Semen Preservation |
| الوصف: | To enhance the conservation of endangered populations, the present study aimed to evaluate whether Tigrinas (Leopardus tigrinus) sperm could be conserved under refrigeration for short periods while maintaining sufficient quality for use in assisted-reproductive techniques (i.e., cryopreservation, in vitro fertilization). For this purpose, semen samples from 15 Tigrinas individuals were submitted to conventional and functional tests after different cooling periods (4 °C; 0, 12, and 24 hours postcooling), using TCM 199 (TCM), Ham's F10 (HAM), Ham's F10 with bovine serum albumin (HBSA), and Tris-citrate egg yolk (TEYC) extenders. In a second step, semen cooled using TEYC was supplemented with reduced glutathione (GSH) at different concentrations (0, 0.5, 1.0, and 1.5 mM). TEYC yielded superior results compared with TCM, HAM, and HBSA even after 24 hours of cooling in regard to the sperm motility index (SMI–TEYC: 50.2 ± 1.7%), high mitochondrial activity (TEYC: 51.4 ± 1.9%), plasma membrane integrity (TEYC: 53 ± 2.1%), and DNA integrity (TEYC: 56.3 ± 2.9%). In regard to the concentration of thiobarbituric-acid-reactive substances (TBARS), TEYC (1900.1 ± 341.4 ng/106 spermatozoa) showed higher levels compared with the other extenders (HAM: 638.7 ± 121.6 ng/106 spermatozoa; HBSA: 468.7 ± 95.6 ng/106 spermatozoa; TCM: 169.6 ± 31.6 ng/106 spermatozoa). However, GSH therapy had no effect. In conclusion, the TEYC extender may be useful in maintaining sperm parameters of Tigrinas for up to 24 hours at 4 °C. Furthermore, these results allow the transport of this material at a minimum quality to be further used for artificial insemination, in vitro fertilization, and the development of semen cryopreservation protocols. |
| تدمد: | 0093-691X |
| DOI: | 10.1016/j.theriogenology.2016.10.015 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::460c52f7d2537dedd2d79b024f890751 https://doi.org/10.1016/j.theriogenology.2016.10.015 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....460c52f7d2537dedd2d79b024f890751 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 0093691X |
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| DOI: | 10.1016/j.theriogenology.2016.10.015 |