Human fibroblast alterations induced by low power laser irradiation at the single cell level using confocal microscopy
| العنوان: | Human fibroblast alterations induced by low power laser irradiation at the single cell level using confocal microscopy |
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| المؤلفون: | Panagiotis Handris, Dimitris Kletsas, Eleni Alexandratou, Dido Yova, Spyros Loukas |
| المصدر: | Photochemical & Photobiological Sciences. 1:547-552 |
| بيانات النشر: | Springer Science and Business Media LLC, 2002. |
| سنة النشر: | 2002 |
| مصطلحات موضوعية: | Membrane potential, Microscopy, Confocal, Microscope, Chemistry, Lasers, Confocal, Intracellular pH, Dose-Response Relationship, Radiation, Intracellular Membranes, Fibroblasts, Hydrogen-Ion Concentration, Laser, Calcium in biology, Membrane Potentials, law.invention, Cell biology, Cytosol, law, Confocal microscopy, Humans, Physical and Theoretical Chemistry, Reactive Oxygen Species, Cells, Cultured |
| الوصف: | Low power laser irradiation is regarded to have a significant role in triggering cellular proliferation and in treating diseases of diverse etiologies. The present work contributes to the understanding of the mechanisms of action by studying low power laser effects in human fibroblasts. Confocal laser scanning microscopy is used for irradiation and observation of the same area of interest allowing the imaging of laser effects at the single cell level and in real time. Coverslip cultures were placed in a small incubation chamber for in vivo microscopic observation. Laser stimulation of the cells was performed using the 647 nm line of the confocal laser through the objective lens of the microscope. Mitochondrial membrane potential (delta psi(m)), intracellular pH, calcium alterations and generation of reactive oxygen species (ROS) were monitored using specific fluorescent vital probes. The induced effects were quantified using digital image processing techniques. After laser irradiation, a gradual alkalinization of the cytosolic pH and an increase in mitochondrial membrane potential were observed. Recurrent spikes of intracellular calcium concentration were also triggered by laser. Reactive oxygen species were generated as a result of biostimulation. No such effects were monitored in microscopic fields other than the irradiated ones. |
| تدمد: | 1474-9092 1474-905X |
| DOI: | 10.1039/b110213n |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::45f4c39d0a3bcebb9aae1603284ee048 https://doi.org/10.1039/b110213n |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....45f4c39d0a3bcebb9aae1603284ee048 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14749092 1474905X |
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| DOI: | 10.1039/b110213n |