Cross-tissue organization of the fibroblast lineage
| العنوان: | Cross-tissue organization of the fibroblast lineage |
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| المؤلفون: | Yeqing Angela Yang, Christian Cox, Matthew B. Buechler, Zora Modrusan, Merone Roose-Girma, Rachana N. Pradhan, Roger Caothien, Richard Bourgon, Sören Müller, Amber W. Wang, Akshay T. Krishnamurty, Shannon J. Turley, Aslihan Karabacak Calviello, Joseph R. Arron, Lucinda Tam |
| المصدر: | Nature. 593:575-579 |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Lineage (genetic), Inflammation, Biology, medicine.disease_cause, Autoimmunity, Transcriptome, Mice, 03 medical and health sciences, 0302 clinical medicine, Fibrosis, Neoplasms, medicine, Animals, Humans, Disease, Gene Knock-In Techniques, RNA-Seq, Fibroblast, Cells, Cultured, Multidisciplinary, Cancer, Fibroblasts, medicine.disease, Phenotype, Cell biology, Mice, Inbred C57BL, 030104 developmental biology, medicine.anatomical_structure, Organ Specificity, Female, Single-Cell Analysis, Stromal Cells, medicine.symptom, 030217 neurology & neurosurgery |
| الوصف: | Fibroblasts are non-haematopoietic structural cells that define the architecture of organs, support the homeostasis of tissue-resident cells and have key roles in fibrosis, cancer, autoimmunity and wound healing1. Recent studies have described fibroblast heterogeneity within individual tissues1. However, the field lacks a characterization of fibroblasts at single-cell resolution across tissues in healthy and diseased organs. Here we constructed fibroblast atlases by integrating single-cell transcriptomic data from about 230,000 fibroblasts across 17 tissues, 50 datasets, 11 disease states and 2 species. Mouse fibroblast atlases and a DptIRESCreERT2 knock-in mouse identified two universal fibroblast transcriptional subtypes across tissues. Our analysis suggests that these cells can serve as a reservoir that can yield specialized fibroblasts across a broad range of steady-state tissues and activated fibroblasts in disease. Comparison to an atlas of human fibroblasts from perturbed states showed that fibroblast transcriptional states are conserved between mice and humans, including universal fibroblasts and activated phenotypes associated with pathogenicity in human cancer, fibrosis, arthritis and inflammation. In summary, a cross-species and pan-tissue approach to transcriptomics at single-cell resolution has identified key organizing principles of the fibroblast lineage in health and disease. Single-cell and genetic tools are used to characterize the diversity of fibroblasts across healthy and perturbed tissues in mice and humans. |
| تدمد: | 1476-4687 0028-0836 |
| DOI: | 10.1038/s41586-021-03549-5 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::43cd94afe9f708264d32b72292b58008 https://doi.org/10.1038/s41586-021-03549-5 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....43cd94afe9f708264d32b72292b58008 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14764687 00280836 |
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| DOI: | 10.1038/s41586-021-03549-5 |