Antiviral Role of IFITM Proteins in Classical Swine Fever Virus Infection
| العنوان: | Antiviral Role of IFITM Proteins in Classical Swine Fever Virus Infection |
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| المؤلفون: | Yifan Wang, Wang Dong, Yaru Liu, Cheng Li, Yanming Zhang, Hongqing Zheng, Liang Zhang |
| المصدر: | Viruses, Vol 11, Iss 2, p 126 (2019) Viruses Volume 11 Issue 2 |
| بيانات النشر: | MDPI AG, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Endosome, Swine, 030106 microbiology, Cell, lcsh:QR1-502, Immunofluorescence, Real-Time Polymerase Chain Reaction, Virus Replication, classical swine fever virus, Virus, Article, lcsh:Microbiology, Cell Line, 03 medical and health sciences, interferon-stimulated genes, Virology, Macrophages, Alveolar, IFITM proteins, medicine, Animals, biology, medicine.diagnostic_test, LAMP1, Host Microbial Interactions, Colocalization, Membrane Proteins, RNA-Binding Proteins, Virus Internalization, biology.organism_classification, Antigens, Differentiation, 030104 developmental biology, Infectious Diseases, medicine.anatomical_structure, Classical swine fever, Cytoplasm, endosomes, Interferons |
| الوصف: | The proteins IFITM1, IFITM2, and IFITM3 are host effectors against a broad range of RNA viruses whose roles in classical swine fever virus (CSFV) infection had not yet been reported. We investigated the effect of these proteins on CSFV replication in mammalian cells. The proteins were overexpressed and silenced using lentiviruses. Confocal microscopy was used to determine the distribution of these proteins in the cells, and immunofluorescence colocalization analysis was used to evaluate the relationship between IFITMs and the CSFV endosomal pathway, including early endosomes, late endosomes, and lysosomes. IFITM1, IFITM2, or IFITM3 overexpression significantly inhibited CSFV replication, whereas protein knockdown enhanced CSFV replication. In porcine alveolar macrophages (PAMs), IFITM1 was mainly located at the cell surface, whereas IFITM2 and IFITM3 were mainly located in the cytoplasm. Following CSFV infection, the distribution of IFITM1 changed. IFITM1, IFITM2, and IFITM3 colocalization with Lamp1, IFITM2 with Rab5 and Rab7, and IFITM3 with Rab7 were observed in CSFV-infected cells. Collectively, these results provide insights into the possible mechanisms associated with the anti-CSFV action of the IFITM family. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1999-4915 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::436e94f400af31d9b6df7efe6a6548d0 https://www.mdpi.com/1999-4915/11/2/126 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....436e94f400af31d9b6df7efe6a6548d0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19994915 |
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