Global changes in nuclear positioning of genes and intra- and inter-domain genomic interactions that orchestrate B cell fate
| العنوان: | Global changes in nuclear positioning of genes and intra- and inter-domain genomic interactions that orchestrate B cell fate |
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| المؤلفون: | Kazuko Miyazaki, Vivek Chandra, Sven Heinz, Masaki Miyazaki, Robert Månsson, Claudia Bossen, Christopher Benner, Christopher K. Glass, Cornelis Murre, Yin C. Lin |
| المصدر: | Nature immunology |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | CCCTC-Binding Factor, Transcription, Genetic, Immunology, P300-CBP Transcription Factors, Biology, DNA-binding protein, Article, Mice, Transcription (biology), Proto-Oncogene Proteins, medicine, Basic Helix-Loop-Helix Transcription Factors, Immunology and Allergy, Animals, Cell Lineage, p300-CBP Transcription Factors, Promoter Regions, Genetic, Transcription factor, Cells, Cultured, Genetics, Cell Nucleus, Histone Acetyltransferase p300, B-Lymphocytes, Nuclear Lamina, Lymphopoiesis, Precursor Cells, B-Lymphoid, Gene Expression Regulation, Developmental, Cell biology, DNA-Binding Proteins, Mice, Inbred C57BL, Repressor Proteins, Cell nucleus, medicine.anatomical_structure, CTCF, Trans-Activators, Nuclear lamina |
| الوصف: | The genome is folded into domains located in compartments that are either transcriptionally inert or transcriptionally permissive. Here we used genome-wide strategies to characterize domains during B cell development. Structured interaction matrix analysis showed that occupancy by the architectural protein CTCF was associated mainly with intradomain interactions, whereas sites bound by the histone acetyltransferase p300 or the transcription factors E2A or PU.1 were associated with intra- and interdomain interactions that are developmentally regulated. We identified a spectrum of genes that switched nuclear location during early B cell development. In progenitor cells, the transcriptionally inactive locus encoding early B cell factor (Ebf1) was sequestered at the nuclear lamina, which thereby preserved their multipotency. After development into the pro-B cell stage, Ebf1 and other genes switched compartments to establish new intra- and interdomain interactions associated with a B lineage-specific transcription signature. |
| اللغة: | English |
| تدمد: | 1529-2916 1529-2908 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::42111197abe49bbd5e17b087c973dc85 http://europepmc.org/articles/PMC3501570 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....42111197abe49bbd5e17b087c973dc85 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15292916 15292908 |
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