Lack of vascular endothelial growth factor receptor-2/Flk1 signaling does not affect substantia nigra development
| العنوان: | Lack of vascular endothelial growth factor receptor-2/Flk1 signaling does not affect substantia nigra development |
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| المؤلفون: | Kai F. Loewenbrück, Änne Boldt, Georg Breier, Joanna Kalucka, Johannes Schwarz, Martina Maisel, Christiane Fiedler, Alexander Storch, Andreas Hermann |
| المصدر: | Neuroscience letters 553, 142-147 (2013). doi:10.1016/j.neulet.2013.08.031 Hermann, A, Loewenbrück, K F, Boldt, Ä, Fiedler, C, Maisel, M, Kalucka, J, Schwarz, J, Breier, G & Storch, A 2013, ' Lack of vascular endothelial growth factor receptor-2/Flk1 signaling does not affect substantia nigra development ', Neuroscience Letters, vol. 553, pp. 142-7 . https://doi.org/10.1016/j.neulet.2013.08.031 |
| بيانات النشر: | Elsevier Science, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | medicine.medical_specialty, Cell Survival, Neurogenesis, Cre recombinase, Substantia nigra, Mice, Transgenic, Biology, chemistry.chemical_compound, Mice, Internal medicine, embryology [Substantia Nigra], medicine, metabolism [Substantia Nigra], Animals, ddc:610, Dopaminergic Neurons/cytology, Vascular Endothelial Growth Factor Receptor-2/genetics, cytology [Dopaminergic Neurons], Dopaminergic Neurons, General Neuroscience, Substantia Nigra/embryology, Dopaminergic, Kinase insert domain receptor, Vascular Endothelial Growth Factor Receptor-2, Oxygen tension, Cell biology, Substantia Nigra, Vascular endothelial growth factor, metabolism [Vascular Endothelial Growth Factor Receptor-2], Endocrinology, growth & development [Substantia Nigra], nervous system, chemistry, genetics [Vascular Endothelial Growth Factor Receptor-2], Signal transduction, Signal Transduction |
| الوصف: | Oxygen tension is critical for proliferation of human and murine midbrain-derived neural precursor cells (mNPCs). Lack of hypoxia-inducible factor-1α (HIF1α) impairs midbrain dopaminergic neurogenesis which could be rescued by vascular endothelial growth factor (VEGF) via VEGFR-2 signaling. Here, we conditionally inactivated the VEGFR-2, encoded by the fetal liver kinase 1 (Flk1) gene, in murine NPCs to determine its role in proliferation and survival in vitro as well as survival of dopaminergic neurons in vivo. Flk1 conditional knock-out (Flk1 CKO) mice showed no general brain phenotype. There was no midbrain-specific impairment of NPC proliferation as seen in HIF1α CKO mice. In the substantia nigra (SN) of adult Flk1 CKO mice, nonbiased stereological cell counts revealed no reduction of TH-positive neurons of Flk1 CKO mice compared with control Cre/wt mice (in which the wild-type Flk1 allele is expressed in parallel with the Cre recombinase allele). In conclusion, VEGF receptor signaling seems not to be relevant to the development and survival of substantia nigra dopaminergic neurons within the hypoxia-HIF1α signaling pathway. |
| اللغة: | English |
| DOI: | 10.1016/j.neulet.2013.08.031 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::40991bd0195e38affd48a6ddbcecfaea https://pub.dzne.de/record/137087 |
| Rights: | RESTRICTED |
| رقم الانضمام: | edsair.doi.dedup.....40991bd0195e38affd48a6ddbcecfaea |
| قاعدة البيانات: | OpenAIRE |
| DOI: | 10.1016/j.neulet.2013.08.031 |
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