التفاصيل البيبلوغرافية
| العنوان: |
Obp56h Modulates Mating Behavior in Drosophila melanogaster |
| المؤلفون: |
Logan J. Everett, Trudy F. C. Mackay, Gunjan H. Arya, Genevieve St. Armour, Robert R. H. Anholt, Lavanya Turlapati, John R. Shorter, Lauren M. Dembeck, Coby Schal, Tatiana V. Morozova |
| المصدر: |
G3: Genes, Genomes, Genetics, Vol 6, Iss 10, Pp 3335-3342 (2016)
G3: Genes|Genomes|Genetics |
| بيانات النشر: |
The University of North Carolina at Chapel Hill University Libraries, 2016. |
| سنة النشر: |
2016 |
| مصطلحات موضوعية: |
Male, 0301 basic medicine, Odorant binding, Olfaction, Investigations, QH426-470, Receptors, Odorant, odorant binding protein, Animals, Genetically Modified, Sexual Behavior, Animal, pheromone, 03 medical and health sciences, Copulation, Genetics, Animals, Drosophila Proteins, Metabolomics, Mating, Molecular Biology, Genetics (clinical), Regulation of gene expression, Gene knockdown, biology, Gene Expression Profiling, fungi, FlyBook, biology.organism_classification, Hydrocarbons, 5-tricosene, Drosophila melanogaster, 030104 developmental biology, Gene Expression Regulation, Gene Knockdown Techniques, Odorant-binding protein, biology.protein, cuticular hydrocarbon, Female, RNA Interference, Psychomotor Performance, Drosophila Protein, Genome-Wide Association Study, olfaction |
| الوصف: |
Social interactions in insects are driven by conspecific chemical signals that are detected via olfactory and gustatory neurons. Odorant binding proteins (Obps) transport volatile odorants to chemosensory receptors, but their effects on behaviors remain poorly characterized. Here, we report that RNAi knockdown of Obp56h gene expression in Drosophila melanogaster enhances mating behavior by reducing courtship latency. The change in mating behavior that results from inhibition of Obp56h expression is accompanied by significant alterations in cuticular hydrocarbon (CHC) composition, including reduction in 5-tricosene (5-T), an inhibitory sex pheromone produced by males that increases copulation latency during courtship. Whole genome RNA sequencing confirms that expression of Obp56h is virtually abolished in Drosophila heads. Inhibition of Obp56h expression also affects expression of other chemoreception genes, including upregulation of lush in both sexes and Obp83ef in females, and reduction in expression of Obp19b and Or19b in males. In addition, several genes associated with lipid metabolism, which underlies the production of cuticular hydrocarbons, show altered transcript abundances. Our data show that modulation of mating behavior through reduction of Obp56h is accompanied by altered cuticular hydrocarbon profiles and implicate 5-T as a possible ligand for Obp56h. |
| اللغة: |
English |
| DOI: |
10.17615/gqfh-nm28 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::400fd8d852ed055602bce7fd662f0016 |
| Rights: |
OPEN |
| رقم الانضمام: |
edsair.doi.dedup.....400fd8d852ed055602bce7fd662f0016 |
| قاعدة البيانات: |
OpenAIRE |