Cinnamate‐CoA ligase is involved in biosynthesis of benzoate‐derived biphenyl phytoalexin in Malus × domestica ‘Golden Delicious’ cell cultures
| العنوان: | Cinnamate‐CoA ligase is involved in biosynthesis of benzoate‐derived biphenyl phytoalexin in Malus × domestica ‘Golden Delicious’ cell cultures |
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| المؤلفون: | Kiran Ambatipudi, Javid Iqbal Mir, Partha Pratim Roy, Pawan Kumar Agrawal, Mariam Gaid, Ludger Beerhues, Deepa Teotia, Ragothaman M. Yennamalli, Satyajeet P. Khare, Till Beuerle, Aparna Verma, Shashank Sagar Saini, Debabrata Sircar, Robert Hänsch |
| المصدر: | The Plant Journal. 100:1176-1192 |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Models, Molecular, 0106 biological sciences, 0301 basic medicine, endocrine system, Malus, Protein Conformation, Rosaceae, Cell Culture Techniques, Nicotiana benthamiana, Plant Science, Genes, Plant, Benzoates, 01 natural sciences, Cinnamic acid, Ligases, 03 medical and health sciences, chemistry.chemical_compound, Ascomycota, Gene Expression Regulation, Plant, Phytoalexins, Tobacco, Genetics, Amino Acid Sequence, Plant Diseases, chemistry.chemical_classification, biology, Phytoalexin, Biphenyl Compounds, fungi, Venturia inaequalis, Cell Biology, biology.organism_classification, Elicitor, Molecular Docking Simulation, Plant Leaves, 030104 developmental biology, chemistry, Biochemistry, Cinnamates, Sequence Alignment, Sesquiterpenes, Malinae, 010606 plant biology & botany |
| الوصف: | Apple (Malus sp.) and other genera belonging to the sub-tribe Malinae of the Rosaceae family produce unique benzoic acid-derived biphenyl phytoalexins. Cell cultures of Malus domestica cv. 'Golden Delicious' accumulate two biphenyl phytoalexins, aucuparin and noraucuparin, in response to the addition of a Venturia inaequalis elicitor (VIE). In this study, we isolated and expressed a cinnamate-CoA ligase (CNL)-encoding sequence from VIE-treated cell cultures of cv. 'Golden Delicious' (M. domestica CNL; MdCNL). MdCNL catalyses the conversion of cinnamic acid into cinnamoyl-CoA, which is subsequently converted to biphenyls. MdCNL failed to accept benzoic acid as a substrate. When scab-resistant (cv. 'Shireen') and moderately scab-susceptible (cv. 'Golden Delicious') apple cultivars were challenged with the V. inaequalis scab fungus, an increase in MdCNL transcript levels was observed in internodal regions. The increase in MdCNL transcript levels could conceivably correlate with the pattern of accumulation of biphenyls. The C-terminal signal in the MdCNL protein directed its N-terminal reporter fusion to peroxisomes in Nicotiana benthamiana leaves. Thus, this report records the cloning and characterisation of a cinnamoyl-CoA-forming enzyme from apple via a series of in vivo and in vitro studies. Defining the key step of phytoalexin formation in apple provides a biotechnological tool for engineering elite cultivars with improved resistance. |
| تدمد: | 1365-313X 0960-7412 |
| DOI: | 10.1111/tpj.14506 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3fed4998732f0eebb4ba8eae8f62280c https://doi.org/10.1111/tpj.14506 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3fed4998732f0eebb4ba8eae8f62280c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1365313X 09607412 |
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| DOI: | 10.1111/tpj.14506 |