Preliminary Investigation about the Expression of G Protein-Coupled Receptors in Platelets from Patients with Chronic Immune Thrombocytopenic Purpura
| العنوان: | Preliminary Investigation about the Expression of G Protein-Coupled Receptors in Platelets from Patients with Chronic Immune Thrombocytopenic Purpura |
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| المؤلفون: | Xian-Ru Xu, Cheng-Wei Xu, Long Xie, Chun-Ling He, Duanxu Wang, Wen-Jun Yu, Da-Ming Xu, Jun Yin, Ze-Wen Zhang, Jin-Feng Qiu |
| المصدر: | Acta Haematologica. 144:551-559 |
| بيانات النشر: | S. Karger AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Adult, Blood Platelets, Male, medicine.medical_specialty, Adrenergic receptor, Receptors, G-Protein-Coupled, Flow cytometry, chemistry.chemical_compound, P2Y12, Internal medicine, medicine, Humans, Platelet, Receptor, Purpura, Thrombocytopenic, Idiopathic, medicine.diagnostic_test, business.industry, Hematology, General Medicine, Middle Aged, medicine.disease, Thrombocytopenic purpura, Adenosine diphosphate, Endocrinology, Gene Expression Regulation, chemistry, Chronic Disease, Female, Signal transduction, business, Signal Transduction |
| الوصف: | Objective: The objective of this study was to determine the expression of G protein-coupled receptors (GPCRs) in platelets from adult patients with chronic immune thrombocytopenic purpura (ITP). Methods: Peripheral blood samples were collected from 40 patients with chronic ITP in the Second Affiliated Hospital of Shantou University Medical College, and 40 peripheral blood samples from healthy volunteers were collected; expressions of the adenosine diphosphate receptors (P2Y1 and P2Y12), alpha-2A adrenergic receptor (α2A-AR), and thromboxane A2 receptor (TP) in platelets were detected by flow cytometry. Gα protein, protease-activated receptor 1 (PAR1), and protease-activated receptor 4 (PAR4) were analyzed by Western blot and analyzed statistically. Results: Flow cytometry measurements of mean fluorescence intensities showed platelets from patients with chronic ITP, compared to healthy individuals, had significantly higher levels of P2Y1 (31.4 ± 2.2 vs. 7.8 ± 0.8), P2Y12 (29.6 ± 2.1 vs. 7.2 ± 1.3), α2A-AR (25.8 ± 2.9 vs. 9.8 ± 0.9), and TP (39.8 ± 3.1 vs. 4.7 ± 0.6) (all p < 0.01). Similarly, integrated optical density analysis of Western blots showed that platelets from patients with chronic ITP had significantly higher levels of Gα (1046.3 ± 159.96 vs. 254.49 ± 39.51), PAR1 (832.98 ± 98.81 vs. 203.92 ± 27.47), and PAR4 (1518.80 ± 272.45 vs. 431.27 ± 41.86) (all p < 0.01). Conclusion: Expression of GPCRs is increased in platelets from patients with chronic ITP, suggesting that platelets of chronic ITP may participate in the complicated biological process by means of GPCR-mediated signaling pathways. |
| تدمد: | 1421-9662 0001-5792 |
| DOI: | 10.1159/000514907 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3f0c1f9d6969692d29c2b3169ba3b5b2 https://doi.org/10.1159/000514907 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....3f0c1f9d6969692d29c2b3169ba3b5b2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14219662 00015792 |
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| DOI: | 10.1159/000514907 |