Local Inflammatory Response after Intramuscularly Implantation of Anti-Adhesive Plasma-Fluorocarbon-Polymer Coated Ti6AI4V Discs in Rats
| العنوان: | Local Inflammatory Response after Intramuscularly Implantation of Anti-Adhesive Plasma-Fluorocarbon-Polymer Coated Ti6AI4V Discs in Rats |
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| المؤلفون: | Michael Schlosser, Uwe Walschus, Maciej Patrzyk, Andreas Hoene, Charlotte Koppe, Barbara Nebe, Nico Brandt, Birgit Finke, Christopher Pohl, Jürgen Meichsner, Holger Testrich |
| المصدر: | Polymers Polymers, Vol 13, Iss 2684, p 2684 (2021) Volume 13 Issue 16 |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | titanium (Ti) alloys, Polymers and Plastics, Organic chemistry, Inflammation, Bone healing, Article, chemistry.chemical_compound, inflammatory/immunological response, Immune system, QD241-441, MHC class I, medicine, IL-2 receptor, low-temperature plasma polymerization, anti-adhesive surface, biology, Chemistry, CD68, Octafluoropropane, General Chemistry, Molecular biology, intramuscularly implantation, biology.protein, medicine.symptom, CD163, plasma-fluorocarbon-polymer |
| الوصف: | Orthopaedic implants and temporary osteosynthesis devices are commonly based on Titanium (Ti). For short-term devices, cell-material contact should be restricted for easy removal after bone healing. This could be achieved with anti-adhesive plasma-fluorocarbon-polymer (PFP) films created by low-temperature plasma processes. Two different PFP thin film deposition techniques, microwave (MW) and radiofrequency (RF) discharge plasma, were applied to receive smooth, hydrophobic surfaces with octafluoropropane (C3F8) or hexafluorohexane (C6F6) as precursors. This study aimed at examining the immunological local tissue reactions after simultaneous intramuscular implantation of four different Ti samples, designated as MW-C3F8, MW-C6F6, RF-C3F8 and Ti-controls, in rats. A differentiated morphometric evaluation of the inflammatory reaction was conducted by immunohistochemical staining of CD68+ macrophages, CD163+ macrophages, MHC class II-positive cells, T lymphocytes, CD25+ regulatory T lymphocytes, NK cells and nestin-positive cells in cryosections of surrounding peri-implant tissue. Tissue samples were obtained on days 7, 14 and 56 for investigating the acute and chronical inflammation (n = 8 rats/group). Implants with a radiofrequency discharge plasma (RF-C3F8) coating exhibited a favorable short- and long-term immune/inflammatory response comparable to Ti-controls. This was also demonstrated by the significant decrease in pro-inflammatory CD68+ macrophages, possibly downregulated by significantly increasing regulatory T lymphocytes. |
| وصف الملف: | application/pdf |
| تدمد: | 2073-4360 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3d987c73def5eda1121dc627673f89c2 https://pubmed.ncbi.nlm.nih.gov/34451224 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3d987c73def5eda1121dc627673f89c2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20734360 |
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