Whither metabolic flux analysis in plants?
| العنوان: | Whither metabolic flux analysis in plants? |
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| المؤلفون: | R. George Ratcliffe, Nicholas J. Kruger |
| المصدر: | Journal of Experimental Botany |
| بيانات النشر: | Oxford University Press (OUP), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | AcademicSubjects/SCI01210, Flux balance analysis, Physiology, Stable isotope ratio, Chemistry, Metabolic network, steady-state analysis, Plant Science, Non stationary analysis, Plants, eXtra Botany, Models, Biological, Metabolic pathway, Viewpoint, metabolic flux analysis, metabolic network, Metabolic flux analysis, non-stationary analysis, metabolic phenotype, Metabolic phenotype, Biological system, Metabolic Networks and Pathways, Organism |
| الوصف: | Stable isotope labelling experiments provide many opportunities for probing metabolic pathways. One goal of such experiments is to define the metabolic phenotype of an organism in terms of the fluxes supported by the entire metabolic network. Metabolic flux analysis (MFA) is used routinely in prokaryotes, but its application to plants is more challenging. Here we examine the status of MFA in plants, highlighting difficulties that hinder wider exploitation of the technique. We conclude that simulation of network fluxes using constraints-based modelling offers a more versatile approach for exploring the capabilities of a network, and that MFA might be best used to probe the interesting features that simulation reveals. |
| تدمد: | 1460-2431 0022-0957 |
| DOI: | 10.1093/jxb/erab389 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3cfab38629472e09385e7e4d5ab0b3fd https://doi.org/10.1093/jxb/erab389 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3cfab38629472e09385e7e4d5ab0b3fd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14602431 00220957 |
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| DOI: | 10.1093/jxb/erab389 |