Antioxidant CR-6 Protects against Reperfusion Injury after a Transient Episode of Focal Brain Ischemia in Rats
| العنوان: | Antioxidant CR-6 Protects against Reperfusion Injury after a Transient Episode of Focal Brain Ischemia in Rats |
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| المؤلفون: | Roser Gorina, Francesc Jiménez-Altayó, Ángel Chamorro, Emili Martínez, Fernando J. Pérez-Asensio, Angel Messeguer, Elisabet Vila, Xavier de la Rosa, Anna M. Planas |
| المصدر: | Journal of Cerebral Blood Flow & Metabolism Digital.CSIC. Repositorio Institucional del CSIC instname Journal of Cerebral Blood Flow & Metabolism; Vol 30 |
| بيانات النشر: | SAGE Publications, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Male, Brain Edema, medicine.disease_cause, Antioxidants, Brain Ischemia, Rats, Sprague-Dawley, Brain ischemia, 0302 clinical medicine, Leukocytes, Stroke, 0303 health sciences, Cerebral infarction, Infarction, Middle Cerebral Artery, Cerebral Infarction, Immunohistochemistry, Reactive Nitrogen Species, 3. Good health, Neuroprotective Agents, Neutrophil Infiltration, Neurology, Blood-Brain Barrier, Cerebrovascular Circulation, Reperfusion Injury, Anesthesia, Middle cerebral artery, Original Article, Cardiology and Cardiovascular Medicine, Blotting, Western, Ischemia, Hyperemia, 03 medical and health sciences, medicine.artery, medicine, Animals, Benzopyrans, cardiovascular diseases, Reactive hyperemia, 030304 developmental biology, Inflammation, business.industry, medicine.disease, Rats, Oxidative Stress, Cyclooxygenase 2, RNA, Neurology (clinical), business, Reperfusion injury, Psychomotor Performance, 030217 neurology & neurosurgery, Oxidative stress |
| الوصف: | Oxidative and nitrosative stress are targets for intervention after ischemia/reperfusion. The aim of this study was to explore the effect of CR-6, a vitamin-E analogue that is antioxidant and scavenger of nitrogen-reactive species. Sprague–Dawley rats had the middle cerebral artery (MCA) occluded either for 90 mins or permanently. Cortical perfusion was continuously monitored by laser–Doppler flowmetry. CR-6 (100 mg/kg) was administered orally either at 2 and 8 h after MCA occlusion, or at 2 h only. Infarct volume, neurological deficit, and signs of reperfusion injury were evaluated. CR-6 was detected in plasma and brain by HPLC. CR-6 reduced glutathione consumption in the ischemic brain and superoxide generation in the isolated MCA. CR-6 decreased infarct volume and attenuated the neurological deficit at 1 and 7 days after ischemia/reperfusion, but not after permanent ischemia. Immediately after reperfusion, cortical blood flow values returned to their baseline (±20%) in several animals, whereas others showed hyper-perfusion (>20% of baseline). Reactive hyperemia was associated with adverse events such as increased cortical BBB leakage, edema, protein nitrotyrosination, COX-2 expression, and neutrophil accumulation; and with a poorer outcome, and CR-6 attenuated these effects. In conclusion, oral CR-6 administration after transient ischemia protects the brain from reperfusion injury. This study was supported by grants from the Ministerio de Educación y Ciencia (SAF2005-05793-CO1, SAF2008-04515-C01, SAF2007-60406, and SAF2008-00048) and the European Community through the European Network of Excellence DiMI (LSHB-CT-2005-512146) and the FP7/2007-2013 project (grant agreement no. 201024). |
| تدمد: | 1559-7016 0271-678X |
| DOI: | 10.1038/jcbfm.2009.237 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c9bb0c6a425aee9e439708cc134cb4f https://doi.org/10.1038/jcbfm.2009.237 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3c9bb0c6a425aee9e439708cc134cb4f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15597016 0271678X |
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| DOI: | 10.1038/jcbfm.2009.237 |