التفاصيل البيبلوغرافية
| العنوان: |
Fluorescent protein-based detection of unconjugated bilirubin in newborn serum |
| المؤلفون: |
Atsushi Miyawaki, Sachiyo Fukushima, Hajime Nakamura, Hisahide Nishio, Noriyuki Nishimura, Keiji Yamana, Kosuke Nishida, Kazumoto Iijima, Tsubasa Koda, Ichiro Morioka, Daisuke Kurokawa, Sota Iwatani |
| المصدر: |
Scientific Reports |
| بيانات النشر: |
Nature Publishing Group, 2016. |
| سنة النشر: |
2016 |
| مصطلحات موضوعية: |
0301 basic medicine, Fish Proteins, Male, medicine.medical_specialty, Oxidoreductases Acting on CH-CH Group Donors, Bilirubin, Conjugated bilirubin, Article, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, 030225 pediatrics, Internal medicine, medicine, Fluorescent protein, Animals, Humans, Bilirubin oxidase, Multidisciplinary, Eels, Chemistry, Infant, Newborn, Serum samples, Unconjugated bilirubin, Luminescent Proteins, 030104 developmental biology, Endocrinology, Biochemistry, Lipid emulsion, Biological Assay, Female, Hemoglobin |
| الوصف: |
Increased serum levels of unconjugated bilirubin are associated with the development of brain damage in newborns. In current clinical settings, there are no methods for directly determining serum levels of unconjugated bilirubin. UnaG, a fluorescent protein from Japanese eel muscle that specifically binds to unconjugated bilirubin was used in this study. Linear regression analysis was carried out to compare unconjugated bilirubin levels measured by UnaG and conventional bilirubin oxidase methods. Unconjugated bilirubin levels in the serum of newborns who were untreated or treated with phototherapy were compared. Effects of interfering factors in the serum (conjugated bilirubin, hemoglobin and lipid) on unconjugated bilirubin concentration measured by the UnaG method were also evaluated. Unconjugated bilirubin levels measured by the UnaG method were highly correlated with those determined by the bilirubin oxidase assay. Unconjugated bilirubin levels determined by bilirubin oxidase and UnaG assays were similar in serum samples containing conjugated bilirubin. The performance of the UnaG assay was unaffected by phototherapy and the presence of serum hemoglobin and lipid emulsion. These results demonstrate the clinical applicability of the UnaG method for direct measurement of unconjugated bilirubin levels in newborn serum. |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| تدمد: |
2045-2322 |
| DOI: |
10.1038/srep28489 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c6827327336d733b4408818eb95f5cf |
| Rights: |
OPEN |
| رقم الانضمام: |
edsair.doi.dedup.....3c6827327336d733b4408818eb95f5cf |
| قاعدة البيانات: |
OpenAIRE |