6-fluorodopamine selectively destroys neuroblastoma cells expressing the noradrenaline transporter
| العنوان: | 6-fluorodopamine selectively destroys neuroblastoma cells expressing the noradrenaline transporter |
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| المؤلفون: | Hartmut B. Stegmann, Vitaly A. Roginsky, Gabriele Seitz, Dietrich Niethammer, Zyrafete Kuçi, Gernot Bruchelt, Hartwig Wolburg |
| المصدر: | Medical and Pediatric Oncology. 35:612-615 |
| بيانات النشر: | Wiley, 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | Cancer Research, animal structures, Dopamine, Ascorbic Acid, Biology, Neuroblastoma, Norepinephrine, Tumor Cells, Cultured, Extracellular, medicine, Humans, Autologous transplantation, Cytotoxic T cell, Oxidopamine, Cytotoxicity, chemistry.chemical_classification, Reactive oxygen species, Norepinephrine Plasma Membrane Transport Proteins, Symporters, medicine.disease, Cell biology, Gene Expression Regulation, Neoplastic, medicine.anatomical_structure, nervous system, Oncology, Biochemistry, chemistry, Pediatrics, Perinatology and Child Health, Bone marrow, Carrier Proteins, Ex vivo |
| الوصف: | BACKGROUND 6-Hydroxydopamine (6-OHDA) was used for ex vivo purging of bone marrow from neuroblastoma cells before autologous transplantation. However, this concept failed because of the rapid autoxidation of 6-OHDA, which leads to the generation of cytotoxic reactive oxygen species (ROS), mainly in the incubation medium before 6-OHDA can be incorporated by neuroblastoma cells. PROCEDURE We based our experiments on the theory that, in contrast, 6-fluorodopamine (6-FDA), which is slowly converted to 6-OHDA at neutral pH, is able to enter neuroblastoma cells via the noradrenaline transporter (NA-T). Therefore, most ROS are generated inside the target cells. RESULTS Small amounts of ascorbate prevent the extracellular conversion of 6-FDA to 6-OHDA without affecting its cytotoxicity, leading to an even more selective effect of 6-FDA. CONCLUSIONS We conclude that 6-FDA is a promising substance for selective destruction of NA-T-positive neuroblastoma cells. |
| تدمد: | 1096-911X 0098-1532 |
| DOI: | 10.1002/1096-911x(20001201)35:6<612::aid-mpo26>3.0.co;2-u |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3bb18f2b06a4ddfff842dd57bc872d99 https://doi.org/10.1002/1096-911x(20001201)35:6<612::aid-mpo26>3.0.co;2-u |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....3bb18f2b06a4ddfff842dd57bc872d99 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1096911X 00981532 |
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| DOI: | 10.1002/1096-911x(20001201)35:6<612::aid-mpo26>3.0.co;2-u |