DETECTION OF ADENOVIRUS ANTIGEN BY A SURFACE-ENHANCED RAMAN SCATTERING ENZYME-LINKED IMMUNOSORBENT ASSAY
| العنوان: | DETECTION OF ADENOVIRUS ANTIGEN BY A SURFACE-ENHANCED RAMAN SCATTERING ENZYME-LINKED IMMUNOSORBENT ASSAY |
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| المؤلفون: | A. Yu. Olenin, A. M. Kudryashova, O. V. Borisova, G. V. Lisichkin, Zverev Vv, A. G. Galstian, E. B. Faizuloev |
| المصدر: | Журнал микробиологии, эпидемиологии и иммунобиологии, Vol 1, Iss 3, Pp 25-31 (2019) |
| بيانات النشر: | Central Research Institute for Epidemiology, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | chemistry.chemical_classification, silver nanoparticles, genetic structures, viruses, Medicine (miscellaneous), adenovirus, General Medicine, surface-enhanced raman scattering, Microbiology, behavioral disciplines and activities, Molecular biology, QR1-502, 3,3',5,5'-tetramethylbenzidine, symbols.namesake, Enzyme, Adenovirus Antigen, chemistry, symbols, enzyme-linked immunosorbent assay, psychological phenomena and processes, Raman scattering |
| الوصف: | Aim. Study of the possibility of adenovirus antigen detection by recording of surface-enhanced Raman scattering (SERS) spectra of enzyme oxidized product of 3,3',5,5'-tetramethylbenzidine. Materials and methods. Clinical fecal samples containing adenoviruses, group A rotaviruses, noroviruses and healthy children samples, as well as laboratory strains of adenoviruses with a titer of 5 — 6 lg TCD50/ml were used. Sandwich immunoassay was used, the Raman spectra were recorded by a Raman spectrometer (532 nm) after incubation with silver nanoparticles. Results. The concordance of the adenovirus detection results was obtained in comparison with the enzyme immunoassay method with colorimetric detection and PCR. Conclusion. The possibility of TMB+ using as a SERS reporter and silver nanoparticles as a SERS substrate for the detection of adenovirus antigen in complex biological samples was shown. |
| تدمد: | 2686-7613 0372-9311 |
| DOI: | 10.36233/0372-9311-2018-3-25-31 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3b4c4806b9d142e6aac61a00fd5af738 https://doi.org/10.36233/0372-9311-2018-3-25-31 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3b4c4806b9d142e6aac61a00fd5af738 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 26867613 03729311 |
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| DOI: | 10.36233/0372-9311-2018-3-25-31 |