Proteome reference map of the skin mucus of Atlantic cod (Gadus morhua) revealing immune competent molecules
| العنوان: | Proteome reference map of the skin mucus of Atlantic cod (Gadus morhua) revealing immune competent molecules |
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| المؤلفون: | Jan H.W.M. Rombout, Binoy Rajan, Jorge M.O. Fernandes, Monica F. Brinchmann, Christopher Marlowe A. Caipang, Viswanath Kiron |
| المصدر: | Fish and Shellfish Immunology 31 (2011) 2 Fish and Shellfish Immunology, 31(2), 224-231 |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Fish Proteins, Galectin 1, Proteome, Molecular Sequence Data, Celbiologie en Immunologie, Aquatic Science, Biology, Mannose-Binding Lectin, cyclophilin-a, lepeophtheirus-salmonis, Tacrolimus Binding Proteins, Cyclophilin A, Tandem Mass Spectrometry, intestinal mucus, epidermal mucus, Environmental Chemistry, Gadus, Animals, Electrophoresis, Gel, Two-Dimensional, ictalurus-punctatus, Mannan-binding lectin, Skin, Expressed Sequence Tags, cyprinus-carpio, Mucous Membrane, binding-lectin, Reverse Transcriptase Polymerase Chain Reaction, Binding protein, General Medicine, channel catfish, biology.organism_classification, Cystatin B, Proteasome, Biochemistry, Gadus morhua, Cell Biology and Immunology, conger-myriaster, Immunology, WIAS, Atlantic cod, Sequence Alignment, neutrophil elastase, Chromatography, Liquid |
| الوصف: | The skin mucosal proteome of Atlantic cod ( Gadus morhua ) was mapped using a 2D PAGE, LC–MS/MS coupled approach. Mucosal proteins from naive fish were identified primarily by similarity searches across various cod EST databases. The identified proteins were clustered into 8 groups based on gene ontology classification for biological process. Most of the proteins identified from the gel are hitherto unreported for cod. Galectin-1, mannan binding lectin (MBL), serpins, cystatin B, cyclophilin A, FK-506 binding protein, proteasome subunits (alpha-3 and -7), ubiquitin, and g-type lysozyme are considered immune competent molecules. Five of the aforementioned proteins were cloned and their tissue distribution was analysed by RT-PCR. |
| وصف الملف: | application/pdf; application/octet-stream |
| اللغة: | English |
| تدمد: | 1050-4648 |
| DOI: | 10.1016/j.fsi.2011.05.006 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3a6746f7a144096bcb8d524e420e6ed1 https://doi.org/10.1016/j.fsi.2011.05.006 |
| Rights: | RESTRICTED |
| رقم الانضمام: | edsair.doi.dedup.....3a6746f7a144096bcb8d524e420e6ed1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10504648 |
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| DOI: | 10.1016/j.fsi.2011.05.006 |