Perfusion Decellularization of Extrahepatic Bile Duct Allows Tissue-Engineered Scaffold Generation by Preserving Matrix Architecture and Cytocompatibility
| العنوان: | Perfusion Decellularization of Extrahepatic Bile Duct Allows Tissue-Engineered Scaffold Generation by Preserving Matrix Architecture and Cytocompatibility |
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| المؤلفون: | Norma S. Pérez-Gallardo, Yolik Ramírez-Marín, David Eduardo Abad-Contreras, Francisco Drusso Martinez-Garcia, Martin C. Harmsen, Jorge Luna del Villar-Velasco, Lorena Villafuerte-García, Gonzalo Torres-Villalobos, David M Giraldo-Gomez, Jesús Tapia-Jurado, Leonardo A. Rodriguez-Lopez, M. Cristina Piña-Barba, Miguel Angel Mercado, Martha Ustarroz-Cano, Dulce Maria Puente-Guzmán |
| المساهمون: | Restoring Organ Function by Means of Regenerative Medicine (REGENERATE) |
| المصدر: | Materials Materials, Vol 14, Iss 3099, p 3099 (2021) Materials, 14(11):3099. IOP PUBLISHING LTD Volume 14 Issue 11 |
| بيانات النشر: | MDPI, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Technology, Pathology, medicine.medical_specialty, Scaffold, H&E stain, 02 engineering and technology, Matrix (biology), Article, Extracellular matrix, 03 medical and health sciences, Cytokeratin, chemistry.chemical_compound, bile duct, medicine, General Materials Science, DAPI, 030304 developmental biology, matrix scaffold, Microscopy, QC120-168.85, 0303 health sciences, Decellularization, Bile duct, Chemistry, QH201-278.5, Engineering (General). Civil engineering (General), 021001 nanoscience & nanotechnology, extracellular matrix (ECM), TK1-9971, medicine.anatomical_structure, Descriptive and experimental mechanics, decellularization, Electrical engineering. Electronics. Nuclear engineering, TA1-2040, 0210 nano-technology |
| الوصف: | Reconstruction of bile ducts damaged remains a vexing medical problem. Surgeons have few options when it comes to a long segment reconstruction of the bile duct. Biological scaffolds of decellularized biliary origin may offer an approach to support the replace of bile ducts. Our objective was to obtain an extracellular matrix scaffold derived from porcine extrahepatic bile ducts (dECM-BD) and to analyze its biological and biochemical properties. The efficiency of the tailored perfusion decellularization process was assessed through histology stainings. Results from 4’-6-diamidino-2-phenylindole (DAPI), Hematoxylin and Eosin (H& E) stainings, and deoxyribonucleic acid (DNA) quantification showed proper extracellular matrix (ECM) decellularization with an effectiveness of 98%. Immunohistochemistry results indicate an effective decrease in immunogenic marker as human leukocyte antigens (HLA-A) and Cytokeratin 7 (CK7) proteins. The ECM of the bile duct was preserved according to Masson and Herovici stainings. Data derived from scanning electron microscopy (SEM) and thermogravimetric analysis (TGA) showed the preservation of the dECM-BD hierarchical structures. Cytotoxicity of dECM-BD was null, with cells able to infiltrate the scaffold. In this work, we standardized a decellularization method that allows one to obtain a natural bile duct scaffold with hierarchical ultrastructure preservation and adequate cytocompatibility. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1996-1944 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3a4591462e971d74e013ffb428cdaeab http://europepmc.org/articles/PMC8201334 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3a4591462e971d74e013ffb428cdaeab |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19961944 |
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