Plasmodium vivax Sporozoite Production in Anopheles albimanus Mosquitoes for Vaccine Clinical Trials
| العنوان: | Plasmodium vivax Sporozoite Production in Anopheles albimanus Mosquitoes for Vaccine Clinical Trials |
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| المؤلفون: | Hugo Hurtado, Myriam Arévalo-Herrera, Mark A. James, Maria R. Manzano, Sócrates Herrera, Yezid Solarte, Leonardo de Souza Rocha |
| المصدر: | The American Journal of Tropical Medicine and Hygiene. 84:28-34 |
| بيانات النشر: | American Society of Tropical Medicine and Hygiene, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Plasmodium vivax, Mosquito bite, Biology, Apicomplexa, Random Allocation, Anopheles albimanus, Virology, Anopheles, Malaria Vaccines, parasitic diseases, Malaria, Vivax, medicine, Animals, Humans, Articles, medicine.disease, biology.organism_classification, Insect Vectors, Clinical trial, Infectious Diseases, Sporozoites, Female, Parasitology, Plasmodium vivax Malaria, Blood bank, Malaria |
| الوصف: | Vaccine development for Plasmodium vivax malaria is underway. A model to assess the protective efficacy of vaccine candidates in humans is urgently needed. Given the lack of continuous P. vivax cultures, we developed a system to infect Anopheles albimanus mosquitoes using blood from P. vivax-infected patients and determined parameters for challenge of malaria-naive volunteers by mosquito bite. Absence of co-infections in parasitized blood was confirmed by tests consistent with blood bank screening. A total of 119 experiments were conducted using batches of 900–4,500 mosquitoes fed by an artificial membrane feeding method. Optimal conditions for mosquito probing and infection were determined. Presence of oocyst and sporozoites were assessed on Days 7–8 and 14–15, respectively, and conditions to choose batches of infected mosquitoes for sporozoite challenge were established. Procedures to infect volunteers took a 2-hour period including verification of inoculum dose. Anopheles albimanus mosquitoes represent a valuable resource for P. vivax sporozoite challenge of volunteers. |
| تدمد: | 1476-1645 0002-9637 |
| DOI: | 10.4269/ajtmh.2011.09-0499 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::398171ec995b69452c6be1c562c9c876 https://doi.org/10.4269/ajtmh.2011.09-0499 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....398171ec995b69452c6be1c562c9c876 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14761645 00029637 |
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| DOI: | 10.4269/ajtmh.2011.09-0499 |