Importance of local hypoxia on endothelial phenotype for an in vitro approach to bone marrow angiogenesis
| العنوان: | Importance of local hypoxia on endothelial phenotype for an in vitro approach to bone marrow angiogenesis |
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| المؤلفون: | Marc Vasse, Jean-Pierre Vannier, Isabelle Dubus, Sophie Kamanzi, Flore Gouel, Cécile Corbière, Paul Mulder, Elisabeth Legrand, Catherine Buquet |
| المساهمون: | Micro-Environnement et Régulation Cellulaire Intégrée (MERCI), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Aliments Bioprocédés Toxicologie Environnements (ABTE), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU), Service d'hémato-immuno-oncologie pédiatrique [Rouen], CHU Rouen, Institute for Research and Innovation in Biomedicine (IRIB), Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| المصدر: | Leukemia Research Leukemia Research, Elsevier, 2012, 36 (9), pp.1200-1203. ⟨10.1016/j.leukres.2012.05.026⟩ |
| بيانات النشر: | Elsevier BV, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Vascular Endothelial Growth Factor A, Cancer Research, Pathology, Angiogenesis, [SDV]Life Sciences [q-bio], MESH: Cell Hypoxia, Cell Culture Techniques, Endothelial Growth Factors, MESH: Endothelial Growth Factors, 0302 clinical medicine, Bone Marrow, MESH: Endothelial Cells, Angiogenic Proteins, MESH: Models, Theoretical, Receptor, Cells, Cultured, 0303 health sciences, Cell Differentiation, Hematology, Angiopoietin receptor, Cell Hypoxia, 3. Good health, Oxygen tension, Leukemia, Phenotype, medicine.anatomical_structure, Oncology, 030220 oncology & carcinogenesis, [SDE]Environmental Sciences, MESH: Bone Marrow, MESH: Neovascularization, Physiologic, MESH: Oxygen, MESH: Cells, Cultured, MESH: Cell Differentiation, medicine.medical_specialty, MESH: Angiogenic Proteins, Neovascularization, Physiologic, Biology, MESH: Phenotype, 03 medical and health sciences, In vivo, medicine, Humans, 030304 developmental biology, MESH: Cell Culture Techniques, MESH: Humans, MESH: Vascular Endothelial Growth Factor A, Endothelial Cells, Models, Theoretical, medicine.disease, MESH: Receptors, Vascular Endothelial Growth Factor, In vitro, Oxygen, Receptors, Vascular Endothelial Growth Factor, biology.protein, Cancer research, Bone marrow |
| الوصف: | International audience; The vasculature of bone marrow differs from that in other organs, and its characteristics should be considered when exploring the medullar angiogenesis associated with hematological malignancies. We show here that the human bone marrow sinusoidal cell line HBME-1 has a specific expression pattern of angiogenic factors and receptors, characterized by a unique VEGFR3(+), Tie2(-) signature, that resembles the in vivo pattern. Moreover, the HBME-1 cultured for up to 3 days in hypoxic conditions, similar to those found in the bone marrow, specifically downregulated expression of VEGFR1, VEGFR2 and ETAR. Thus, a model using bone marrow sinusoidal cells cultured under reduced oxygen tension may be more relevant than classical in vitro endothelial cultures for understanding the interactions between endothelial and malignant cells in the medullar microenvironment. |
| تدمد: | 0145-2126 |
| DOI: | 10.1016/j.leukres.2012.05.026 |
| DOI: | 10.1016/j.leukres.2012.05.026⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::394d509929bf4c76f6ca501302d11a74 https://doi.org/10.1016/j.leukres.2012.05.026 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....394d509929bf4c76f6ca501302d11a74 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 01452126 |
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| DOI: | 10.1016/j.leukres.2012.05.026 |