ATP depletion induces translocation of STIM1 to puncta and formation of STIM1-ORAI1 clusters: translocation and re-translocation of STIM1 does not require ATP
| العنوان: | ATP depletion induces translocation of STIM1 to puncta and formation of STIM1-ORAI1 clusters: translocation and re-translocation of STIM1 does not require ATP |
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| المؤلفون: | Oleg Vsevolodovich Gerasimenko, Lee P. Haynes, Ciara M. Walsh, Ole H. Petersen, Gyorgy Lur, Svetlana Voronina, Robert D. Burgoyne, Alexei V. Tepikin, Michael Chvanov, Philip S. Rudland, Roger Barraclough |
| المصدر: | Pflugers Archiv Pflugers Archiv : European journal of physiology, vol 457, iss 2 |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Phosphatidylinositol 4,5-Diphosphate, ORAI1 Protein, Physiology, Clinical Biochemistry, Medical Physiology, Calcium-Transporting ATPases, Endoplasmic Reticulum, chemistry.chemical_compound, 0302 clinical medicine, Adenosine Triphosphate, Enzyme Inhibitors, Calcium signaling, Store-operated calcium influx, 0303 health sciences, Microscopy, Microscopy, Confocal, Voltage-dependent calcium channel, ORAI1, ER calcium, STIM1, Cell biology, Neoplasm Proteins, Phosphatidylinositol 4, Protein Transport, Confocal, 5-Diphosphate, Thapsigargin, Protein Binding, inorganic chemicals, Signaling and Cell Physiology, Recombinant Fusion Proteins, Ca2+ signals, Biology, Transfection, 03 medical and health sciences, Physiology (medical), Animals, Humans, Calcium Signaling, Stromal Interaction Molecule 1, 030304 developmental biology, Endoplasmic reticulum, Cell Membrane, Membrane Proteins, Human Movement and Sports Sciences, Rats, ATP, Kinetics, chemistry, nervous system, Hela Cells, Calcium Channels, Adenosine triphosphate, 030217 neurology & neurosurgery, HeLa Cells |
| الوصف: | Depletion of the endoplasmic reticulum (ER) calcium store triggers translocation of stromal interacting molecule one (STIM1) to the sub-plasmalemmal region and formation of puncta-structures in which STIM1 interacts and activates calcium channels. ATP depletion induced the formation of STIM1 puncta in PANC1, RAMA37, and HeLa cells. The sequence of events triggered by inhibition of ATP production included a rapid decline of ATP, depletion of phosphatidylinositol 4,5-bisphosphate (PI(4,5)P(2)) and a slow calcium leak from the ER followed by formation of STIM1 puncta. STIM1 puncta induced by ATP depletion were co-localized with clusters of ORAI1 channels. STIM1-ORAI1 clusters that developed as a result of ATP depletion were very poor mediators of Ca(2+) influx. Re-translocation of STIM1 from puncta back to the ER was observed during total ATP depletion. We can therefore conclude that STIM1 translocation and re-translocation as well as formation of STIM1-ORAI1 clusters occur in an ATP-independent fashion and under conditions of PI(4,5)P(2) depletion. |
| وصف الملف: | application/pdf |
| تدمد: | 0031-6768 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::38e226a18c65be621cb555885f572215 https://pubmed.ncbi.nlm.nih.gov/18542992 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....38e226a18c65be621cb555885f572215 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00316768 |
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