Strain-specific variation in a soilborne phytopathogenic fungus for the expression of genes involved in pH signal transduction pathway, pathogenesis and saprophytic survival in response to environmental pH changes
| العنوان: | Strain-specific variation in a soilborne phytopathogenic fungus for the expression of genes involved in pH signal transduction pathway, pathogenesis and saprophytic survival in response to environmental pH changes |
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| المؤلفون: | Muriel Marchi, Cécile Gracianne, Anne-Yvonne Guillerm-Erckelboudt, Lionel Lebreton, Alain Sarniguet, Stéphanie Daval, Morgane Boutin, Kévin Gazengel |
| المساهمون: | Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, INRA Plant Health and Environment division, Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) |
| المصدر: | Fungal Genetics and Biology Fungal Genetics and Biology, Elsevier, 2013, 61, pp.80-89. ⟨10.1016/j.fgb.2013.09.009⟩ Fungal Genetics and Biology, 2013, 61, pp.80-89. ⟨10.1016/j.fgb.2013.09.009⟩ |
| بيانات النشر: | HAL CCSD, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | [SDV]Life Sciences [q-bio], Genes, Fungal, Laccases, Biology, Microbiology, 03 medical and health sciences, Ascomycota, Stress, Physiological, Gene Expression Regulation, Fungal, Gene expression, Genetics, Gene, Transcription factor, Triticum, 030304 developmental biology, 0303 health sciences, Strain (chemistry), 030306 microbiology, Xylanase, Gene Expression Profiling, pH signalling pathway, Gaeumannomyces graminis var. tritici, Glucanase, Hydrogen-Ion Concentration, Mitogen-activated protein kinase, Biochemistry, Signal transduction, Function (biology), Signal Transduction |
| الوصف: | International audience; The soilborne fungus Gaeumannomyces graminis var. tritici (Ggt) causes take-all, a wheat root disease. In an original strain-specific way, a previous study indicates that inside the Ggt species, some strains grow preferentially at acidic pH and other strains at neutral/alkaline pH. The most important mechanism for a fungal response to the environmental pH is the Pal pathway which integrates the products of the six pal genes and the transcription factor PacC. To evaluate whether the Ggt strain-specific growth in function of the ambient pH is mediated via the Pal pathway, a transcriptional study of the genes encoding this pathway was carried out. This study provided the first evidence that the pH signalling pathway similar to those described in other fungi operated in Ggt. The pacC gene was induced at neutral pH whatever the strain. In an original way, the expression of Ggt genes coding for the different Pal proteins depended on the strain and on the ambient pH. In the strain growing better at acidic pH, few pal genes were pH-regulated, and some were overexpressed at neutral pH when regulated. In the strain growing better at neutral pH, underexpression of most of the pal genes at neutral pH occurred. The strains displayed higher gene expression in the ambient pH that unfavoured their growth as if it was a compensation system. All pH taken together, a globally weaker Pal transcript level occurred in the strains that were less sensitive to acidic pH, and on the contrary, the strain growing better on neutral pH showed higher Pal mRNA levels. The expression of genes involved in pathogenesis and saprophytic growth was also regulated by the ambient pH and the strain: each gene displayed a specific pH-regulation that was similar between strains. But all pH taken together, the global transcript levels of four out of six genes were higher in the strain growing better on neutral pH. Altogether, for the first time, the results show that inside a species, conditions affecting environmental pH modulate the expression of genes in an original strain-specific way. |
| اللغة: | English |
| تدمد: | 1087-1845 1096-0937 |
| DOI: | 10.1016/j.fgb.2013.09.009⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::386ac4013f1edff4bef4603500532666 https://hal.archives-ouvertes.fr/hal-01208686 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....386ac4013f1edff4bef4603500532666 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10871845 10960937 |
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| DOI: | 10.1016/j.fgb.2013.09.009⟩ |