Characterization of the light chain-restricted clonal B cells in peripheral blood of HCV-positive patients
| العنوان: | Characterization of the light chain-restricted clonal B cells in peripheral blood of HCV-positive patients |
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| المؤلفون: | Michitoshi Hashiguchi, Takato Ueno, Rie Imamura, Takashi Okamura, Yuka Takata, Koichi Osaki, Kohji Yoshimoto, Eijiro Oku, Korenori Ohtsubo, Ritsuko Seki, Takumi Kawaguchi, Kazuaki Yakushiji, Taisuke Kanaji, Satoshi Morishige, Michio Sata |
| المصدر: | International Journal of Hematology. 89:452-459 |
| بيانات النشر: | Springer Science and Business Media LLC, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Male, Population, Lymphoproliferative disorders, Biology, CD5 Antigens, Immunoglobulin light chain, CD19, Cell Movement, Interferon, medicine, Humans, education, Aged, Aged, 80 and over, B-Lymphocytes, education.field_of_study, Hematology, Antigens, CD20, medicine.disease, Hepatitis C, Virology, Protein Transport, Gene Expression Regulation, Proto-Oncogene Proteins c-bcl-2, Case-Control Studies, Monoclonal, Splenectomy, biology.protein, Female, CD5, Antibody, Immunoglobulin Heavy Chains, medicine.drug |
| الوصف: | To investigate the association between hepatitis C virus (HCV) and B cell proliferation, we searched for the clonal B cells by flow cytometric analysis of the surface immunoglobulin kappa (kappa):lambda (lambda) light chain ratios of the circulating B (CD19+) cells in 240 HCV-positive patients and 150 negative controls with liver diseases. Clonal B cells with light chain restriction (kappa:lambda ratio3:1 or1:2) were analyzed for CD5 expression and the presence of monoclonal immunoglobulin heavy-chain (IGH) gene rearrangements and the t(14;18) chromosomal translocation. Clonal B cells were detected in 7 cases with HCV (2.9%), but was never detected in the controls (p0.05). Of the 7 cases, all had monoclonal IGH gene rearrangements and one had the t(14;18) chromosomal translocation. These HCV-related clonal B cells are not uniform in the intensity of CD5 expression and showed no increase in the frequencies of CD5+ population compared with non-clonal B cells. No "chronic lymphocytic leukemia-phenotype" cells were found. The loss of clonality was observed in 2 cases treated with interferon and in one case treated with splenectomy. The longitudinal study is required to determine whether these circulating clonal B cells progress to lymphoproliferative disorders in future or not. |
| تدمد: | 1865-3774 0925-5710 |
| DOI: | 10.1007/s12185-009-0301-x |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::382746a9d1b7b5dae3f19f6aba8a7e8f https://doi.org/10.1007/s12185-009-0301-x |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....382746a9d1b7b5dae3f19f6aba8a7e8f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18653774 09255710 |
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| DOI: | 10.1007/s12185-009-0301-x |