التفاصيل البيبلوغرافية
| العنوان: |
TALEN-mediated mutagenesis in zebrafish reveals a role for r-spondin 2 in fin ray and vertebral development |
| المؤلفون: |
Tohru Suzuki, Moe Takeda, Hayato Yokoi, Masaru Matsuda, Yoshiaki Tatsumi |
| المصدر: |
FEBS Letters. (24):4543-4550 |
| بيانات النشر: |
Federation of European Biochemical Societies. Published by Elsevier B.V. |
| مصطلحات موضوعية: |
Molecular Sequence Data, Mutant, Biophysics, Mutagenesis (molecular biology technique), Biology, Biochemistry, TALEN, Structural Biology, Genetics, Skeletogenesis, Animals, Humans, Amino Acid Sequence, Skates, Fish, R-Spondin-2, Molecular Biology, RSPO2, Zebrafish, Fin ray, Transcription activator-like effector nuclease, DNA Restriction Enzymes, Cell Biology, Zebrafish Proteins, Null mutant, biology.organism_classification, Protein Structure, Tertiary, Gene Expression Regulation, Mutagenesis, Mutation, Intercellular Signaling Peptides and Proteins, R-spondin2 |
| الوصف: |
R-spondin (Rspo) encodes a multi-domain protein that modulates the Wnt-signaling pathway. Two distinct rspo2 zebrafish mutants were generated by TALEN-mediated mutagenesis: a null mutant, rspo2(null), lacking all functional domains, and a hypomorphic mutant, rspo2(tsp), lacking the two N-terminal domains. Mutants were analyzed mainly for abnormalities in the skeletal system. Fin ray skeletons were formed normally in the rspo2(tsp) mutants, but were absent from the rspo2(null) mutants. Hypoplasia of the neural/hemal arches and ribs was observed in both mutants. Thus, the two rspo2 mutants help to identify the functions of Rspo2 in skeletogenesis, as well as functional differences among multiple Rspo2 domains. |
| اللغة: |
English |
| تدمد: |
0014-5793 |
| DOI: |
10.1016/j.febslet.2014.10.015 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::37d775e6554abd00ac2515859ae1ba0c |
| Rights: |
OPEN |
| رقم الانضمام: |
edsair.doi.dedup.....37d775e6554abd00ac2515859ae1ba0c |
| قاعدة البيانات: |
OpenAIRE |