Purification and identification of a phytase from fruity bodies of the winter mushroom, Flammulina velutipes
| العنوان: | Purification and identification of a phytase from fruity bodies of the winter mushroom, Flammulina velutipes |
|---|---|
| المؤلفون: | HW Wang, MJ Zhu, TB Ng |
| المصدر: | African Journal of Biotechnology; Vol 10, No 77 (2011); 17845-17852 |
| بيانات النشر: | Academic Journals (Kenya), 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Gel electrophoresis, Mushroom, Chromatography, biology, Sodium, Size-exclusion chromatography, Ion chromatography, chemistry.chemical_element, biology.organism_classification, Applied Microbiology and Biotechnology, Affinity chromatography, chemistry, Genetics, Phytase, Phytase, Flammulina velutipes, Mushroom, Isolation, Agronomy and Crop Science, Molecular Biology, Biotechnology, Flammulina |
| الوصف: | A phytase, with a molecular mass of 14.8 kDa as determined by gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis was isolated from fresh fruiting bodies of the mushroom Flammulina velutipes. The chromatographic procedure used for isolation of the phytase included ion exchange chromatography on DEAE-cellulose, CM-cellulose, Q-Sepharose, affinity chromatography on Affi-gel blue gel and fast protein liquid chromatography-gel filtration on Superdex 75. The enzyme was adsorbed on DEAE-cellulose, unadsorbed on CM-cellulose and Affi-gel blue gel, and adsorbed on QSepharose. It presented an N-terminal sequence different from those of reported fungal phytases. It exhibited maximal activity at about 45°C and it did not manifest drastic fluctuations in activity over the pH of 3 to 9. The phytase presented activity towards a variety of phosphorylated compounds with the following ranking of activity: ATP > fructose-6-phosphate ≈ glucose-6-phosphate > AMP > ADP> β- glycerophosphate > sodium phytate. It did not present antifungal activity.Key words: Phytase, Flammulina velutipes, Mushroom, Isolation. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| ردمك: | 978-1-78451-785-4 1-78451-785-2 |
| تدمد: | 1684-5315 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::379e72d96c8da8cd19ea1669771760ec https://www.ajol.info/index.php/ajb/article/view/98299 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....379e72d96c8da8cd19ea1669771760ec |
| قاعدة البيانات: | OpenAIRE |
| ردمك: | 9781784517854 1784517852 |
|---|---|
| تدمد: | 16845315 |