Import of assembled PTS1 proteins into peroxisomes of the yeast Hansenula polymorpha
| العنوان: | Import of assembled PTS1 proteins into peroxisomes of the yeast Hansenula polymorpha |
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| المؤلفون: | Anne Koek, Marten Veenhuis, Klaas Nico Faber, Ineke Keizer-Gunnink, Ralf van Dijk, Ida J. van der Klei |
| المساهمون: | Groningen Biomolecular Sciences and Biotechnology, Molecular Cell Biology, Groningen Institute for Gastro Intestinal Genetics and Immunology (3GI), Center for Liver, Digestive and Metabolic Diseases (CLDM) |
| المصدر: | Biochimica et Biophysica Acta-Molecular Cell Research, 1591(1-3):PII S0167-4889(02)00274-4, 157-162. ELSEVIER SCIENCE BV |
| بيانات النشر: | ELSEVIER SCIENCE BV, 2002. |
| سنة النشر: | 2002 |
| مصطلحات موضوعية: | Peroxisome-Targeting Signal 1 Receptor, Green Fluorescent Proteins, BIOGENESIS, Receptors, Cytoplasmic and Nuclear, THIOLASE, Biology, PTS 1, OXIDASE, Pichia, Green fluorescent protein, Hansenula polymorpha, PATHWAY, Aldehyde-Ketone Transferases, DIHYDROXYACETONE SYNTHASE, MAMMALIAN-CELLS, Peroxisomes, peroxisome, Molecular Biology, Thiolase, Peroxisomal matrix, TARGETING SIGNAL, Cell Biology, Peroxisome, TRANSPORT, Alcohol oxidase, Alcohol Oxidoreductases, Luminescent Proteins, Cytosol, Biochemistry, METHANOL METABOLISM, PTS1, Dimerization, MATRIX, Biogenesis |
| الوصف: | Previously, Waterham et al. [EMBO J. 12 (1993) 4785] reported that cytosolic oligomeric alcohol oxidase (AO) is not incorporated into peroxisomes after reassembly of the organelles in the temperature-sensitive peroxisome-deficient mutant pex1-6(ts) of Hansenula polymorpha shifted to permissive growth conditions. Here, we show that the failure to import assembled AO protein is not exemplary for other folded proteins because both an artificial peroxisomal matrix protein, PTS1-tagged GFP (GFP.SKL), and the endogenous dimeric PTS1 protein dihydroxyacetone synthase (DHAS) were imported under identical conditions. In vitro receptor-ligand binding studies using immobilised H. polymorpha Pex5p and crude extracts of methanol-induced pex1-6(ts) cells, showed that AO octamers did not interact with the recombinant PTS1 receptor, at conditions that allowed binding of folded GFP.SKL and dimeric DHAS. This shows that import of oligomeric proteins is not a universal pathway for peroxisomal matrix proteins. (C) 2002 Elsevier Science B.V. All rights reserved. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1879-2596 0167-4889 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::36126bdf5e04e88d521f1dc056bb187e https://research.rug.nl/en/publications/68a08c66-94bd-4940-b377-4367cbc41d34 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....36126bdf5e04e88d521f1dc056bb187e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18792596 01674889 |
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