Molecular prevalence of Trypanosoma spp. in wild rodents of Southeast Asia: influence of human settlement habitat
| العنوان: | Molecular prevalence of Trypanosoma spp. in wild rodents of Southeast Asia: influence of human settlement habitat |
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| المؤلفون: | N Thaprathorn, Stéphane Herder, Yannick Chaval, P. Pumhom, Marc Desquesnes, Serge Morand, Sathaporn Jittapalapong, D Pognon, Sarawut Yangtara, C Milocco, Bounneuang Douangboupha |
| المساهمون: | Kasetsart University (KU), Center of Excellence on Agricultural Biotechnology (CAB), Faculty of Veterinary Medicine, Department of Parasitology, Kafkas University, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), National Agriculture and Forestry Research Institute (NAFRI), Centre de Biologie pour la Gestion des Populations (UMR CBGP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Université Montpellier 2 - Sciences et Techniques (UM2), Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), French ANR Biodiversity grant [ANR 07 BDIV 012], French ANR CPES grant [ANR 11 CPEL 002], Thailand International Cooperation Agency (TICA) |
| المصدر: | Epidemiology and Infection Epidemiology and Infection, Cambridge University Press (CUP), 2014, 142 (6), pp.1221-1230. ⟨10.1017/S0950268813002161⟩ Epidemiol Infect |
| بيانات النشر: | Cambridge University Press (CUP), 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Male, Aging, Identification, Niviventer fulvescens, Rodent, Epidemiology, [SDV]Life Sciences [q-bio], habitat, L73 - Maladies des animaux, Santé publique, Rodent Diseases, 0302 clinical medicine, Seroepidemiologic Studies, Prevalence, Trypanosoma lewisi, Asia, Southeastern, 0303 health sciences, Trypanosoma evansi, biology, rodent-borne diseases, Original Papers, Southeast Asia, Musaraigne, Infectious Diseases, rodents, Female, Seasons, L72 - Organismes nuisibles des animaux, Piégeage des animaux, Trypanosoma, Enquête organismes nuisibles, 030231 tropical medicine, Zoology, Animals, Wild, Rodentia, Models, Biological, 03 medical and health sciences, Sex Factors, Maladie de l'homme, Trypanosomiasis, biology.animal, Maxomys, Animals, Humans, Ribosomal DNA, Ecosystem, Transmission des maladies, 030304 developmental biology, Biologie moléculaire, Animal pathogens, Animal sauvage, biology.organism_classification, genomic DNA, Écologie animale, trypanosomes, Rongeur |
| الوصف: | SUMMARYThis study investigated the molecular prevalence of Trypanosoma lewisi and T. evansi in wild rodents from Cambodia, Lao PDR and Thailand. Between 2008 and 2012, rodents (and shrews) were trapped in nine locations and 616 of these were tested using three sets of primers: TRYP1 (amplifying ITS1 of ribosomal DNA of all trypanosomes), TBR (amplifying satellite genomic DNA of Trypanozoon parasites) and LEW1 (amplifying ITS1 of ribosomal DNA of T. lewisi). Based on the size of the PCR products using TRYP1, 17% were positive for T. lewisi and 1·0% positive for Trypanozoon. Results were confirmed by sequencing PCR products and by using more specific primers (LEW1 and TBR). The specificity of TRYP1 primers, however, failed as rodent DNA was amplified in some instances, giving unexpected product sizes. Using LEW1 primers, 13·3% of the samples were confirmed positive for T. lewisi, both by PCR and sequencing. In Thailand, T. lewisi was found in Rattus tanezumi, R. exulans and Berylmys; in Lao PDR, in R. tanezumi and R. exulans, and in Cambodia in R. tanezumi, R. exulans and R. norvegicus. Using TBR, 1·3% of the samples tested positive for Trypanozoon by PCR and sequencing; T. evansi is the only species of the Trypanozoon subgenus possibly present in wild Asian rodents. These results confirmed its presence in rodents from Thailand (R. tanezumi), Lao PDR (R. tanezumi, R. nitidus) and Cambodia (R. tanezumi, Niviventer fulvescens, Maxomys surifer). Based on the information related to rodent trapping, it was found that rodent species trapped in and around human dwellings had a higher prevalence of T. lewisi infection. R. tanezumi and R. exulans, two synanthropic species, were mainly found infected in this habitat suggesting a role as a reservoir and thus a potential source of T. lewisi for human infection. |
| وصف الملف: | application/pdf |
| تدمد: | 1469-4409 0950-2688 |
| DOI: | 10.1017/s0950268813002161 |
| DOI: | 10.1017/S0950268813002161⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::35c9fc2786303a50c1e60f50f4c027f3 https://doi.org/10.1017/s0950268813002161 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....35c9fc2786303a50c1e60f50f4c027f3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14694409 09502688 |
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| DOI: | 10.1017/s0950268813002161 |